验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, mouse liver tissue |
| Positive IHC detected in | mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Caco-2 cells |
Planning an IHC experiment? We recommend our IHCeasy RGC32 Ready-To-Use IHC Kit. RGC32 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19556-1-AP targets RGC32 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 13 open reading frame 15 |
| 别名 | C13orf15, bA157L14.2, RGCC, RGC-32, RGC 32 |
| 计算分子量 | 15 kDa |
| 观测分子量 | 13-15 kDa |
| GenBank蛋白编号 | NM_014059 |
| 基因名称 | RGC32 |
| Gene ID (NCBI) | 28984 |
| RRID | AB_3085580 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H4X1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RGC32, also named as C13orf15, modulates the activity of cell cycle-specific kinases. RGC32 enhances CDC2 activity. RGC32 may contribute to the regulation of the cell cycle. And it inhibits growth of glioma cells by promoting arrest of mitotic progression at the G2/M transition. The antibody can recognize these 2 isoforms of RGC32.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RGC32 antibody 19556-1-AP | Download protocol |
| IHC protocol for RGC32 antibody 19556-1-AP | Download protocol |
| IF protocol for RGC32 antibody 19556-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |