验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, K-562 cells |
| Positive IP detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| ChIP | See 1 publications below |
产品信息
14784-1-AP targets RFX3 in WB, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, ChIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse, rat |
| 免疫原 | RFX3 fusion protein Ag6413 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | regulatory factor X, 3 (influences HLA class II expression) |
| 别名 | Regulatory factor X 3, RFX3, Transcription factor RFX3 |
| 计算分子量 | 84 kDa |
| 观测分子量 | 70-79 kDa |
| GenBank蛋白编号 | BC067778 |
| 基因名称 | RFX3 |
| Gene ID (NCBI) | 5991 |
| RRID | AB_2178917 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P48380 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RFX3 antibody 14784-1-AP | Download protocol |
| IP protocol for RFX3 antibody 14784-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Distinct regulation of hippocampal neuroplasticity and ciliary genes by corticosteroid receptors. | ||
Aging (Albany NY) RNA-seq analysis of the key long noncoding RNAs and mRNAs related to cognitive impairment after cardiac arrest and cardiopulmonary resuscitation. |
