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验证数据展示

  • WB analysis using 12622-1-AP (same clone as 12622-1-PBS)

    WB analysis using 12622-1-AP (same clone as 12622-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12622-1-AP (RFX2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12622-1-PBS in a different storage buffer formulation.

  • IHC staining of rat testis using 12622-1-AP (same clone as 12622-1-PBS)

    IHC staining of rat testis using 12622-1-AP (same clone as 12622-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 12622-1-AP (RFX2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12622-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 12622-1-AP (same clone as 12622-1-PBS)

    IHC staining of mouse testis using 12622-1-AP (same clone as 12622-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12622-1-AP (RFX2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12622-1-PBS in a different storage buffer formulation.

  • IHC staining of rat testis using 12622-1-AP (same clone as 12622-1-PBS)

    IHC staining of rat testis using 12622-1-AP (same clone as 12622-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 12622-1-AP (RFX2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12622-1-PBS in a different storage buffer formulation.

  • IP experiment of THP-1 using 12622-1-AP (same clone as 12622-1-PBS)

    IP experiment of THP-1 using 12622-1-AP (same clone as 12622-1-PBS)

    IP result of anti-RFX2 (IP:12622-1-AP, 4ug; Detection:12622-1-AP 1:1000) with THP-1 cells lysate 1920 ug. This data was developed using the same antibody clone with 12622-1-PBS in a different storage buffer formulation.

RFX2 Polyclonal antibody, PBS Only

RFX2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 12622-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

DNA binding protein RFX2, DNA-binding protein RFX2, Regulatory factor X 2

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

12622-1-PBS targets RFX2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3310

Product name: Recombinant human RFX2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 424-723 aa of BC028579

Sequence: VLPKDKLISLCQCDPILRWMRSCDHILYQALVEILIPDVLRPVPSTLTQAIRNFAKSLEGWLTNAMSDFPQQVIQTKVGVVSAFAQTLRRYTSLNHLAQAARAVLQNTSQINQMLSDLNRVDFANVQEQASWVCQCEESVVQRLEQDFKLTLQQQSSLDQWASWLDSVVTQVLKQHAGSPSFPKAAQQFLLKWSFYSSMVIRDLTLRSAASFGSFHLIRLLYDEYMFYLVEHRVAEATGETPIAVMGEFNDLASLSLTLLDKDDMGDEQRGSEAGPDARSLGEPLVKRERSDPNHSLQGI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 regulatory factor X, 2 (influences HLA class II expression)
别名 DNA binding protein RFX2, DNA-binding protein RFX2, Regulatory factor X 2
计算分子量 723 aa, 80 kDa
观测分子量80 kDa
GenBank蛋白编号BC028579
基因名称 RFX2
Gene ID (NCBI) 5990
RRIDAB_3741832
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP48378
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RFX2 is a key regulator of ciliogenesis in vertebrates, and knockdown of Rfx2 causes defects in neural tube closure and in left-right axis patterning (PMID: 26248850). RFX2 expression is greatly enhanced in adult testis and that RFX2 is equally prominent in highly enriched populations of late pachytene spermatocytes and round spermatids(PMID: 22227339). RFX2 is also required for the development of left-right asymmetry, and motile cilia on RFX2-expressing cells are sufficient to establish adequate fluid flow across the COA to direct normal LR development in mouse (PMID: 22233545).

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