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验证数据展示

  • IHC staining of human tonsillitis using 24289-1-AP (same clone as 24289-1-PBS)

    IHC staining of human tonsillitis using 24289-1-AP (same clone as 24289-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 24289-1-AP (RFTN1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24289-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 24289-1-AP (same clone as 24289-1-PBS)

    WB analysis of HeLa using 24289-1-AP (same clone as 24289-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 24289-1-AP (RFTN1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24289-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 24289-1-AP (same clone as 24289-1-PBS)

    IHC staining of human tonsillitis using 24289-1-AP (same clone as 24289-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24289-1-AP (RFTN1 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24289-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 24289-1-AP (same clone as 24289-1-PBS)

    IHC staining of human tonsillitis using 24289-1-AP (same clone as 24289-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24289-1-AP (RFTN1 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24289-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

RFTN1 Polyclonal antibody, PBS Only

RFTN1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 24289-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

Raftlin, PIG9, PIB10, MIG2, KIAA0084

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

24289-1-PBS targets RFTN1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag17264

Product name: Recombinant human RFTN1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 29-374 aa of BC100667

Sequence: ETKIDVSYEYRFLEFTTLSAAELPGSSAVRLASLRDLPAQLLELYQQGFSLAALHPFVQPTHEREKTPLEHIFRAILIKKTDRSQKTDLHNEGYILELDCCSSLDHPTDQKLIPEFIKKIQEAASQGLKFVGVIPQYHSSVNSAGSSAPVSTANSTEDARDAKNARGDHASLENEKPGTGDVCSAPAGRNQSPEPSSGPRGEVPLAKQPSSPSGEGDGGELSPQGVSKTLDGPESNPLEVHEEPLSGKMEIFTLFNKPKSHQKCRQYYPVTIPLHVSKNGQTVSGLDANWLEHMSDHFRKGGMLVNAVFYLGIVNDSLHGLTDGVFIFEAVSTEDSKTIQGYDAIV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 raftlin, lipid raft linker 1
别名 Raftlin, PIG9, PIB10, MIG2, KIAA0084
计算分子量 578 aa, 63 kDa
观测分子量 63-70 kDa
GenBank蛋白编号BC100667
基因名称 RFTN1
Gene ID (NCBI) 23180
RRIDAB_2879474
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14699
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Raftlin (RFTN1) was originally identified as a major raft protein with molecular mass of 63 kDa in B cells that colocalized with BCR in the lipid raft before and after BCR activation. RFTN1 possesses fatty acylation sites at the N terminus, it predominantly localizes in the cytoplasm in human epithelial cells and monocyte-derived DCs (Mo-DCs) (PMID: 27022195). Human RFTN1 protein estimated to be 63~70 kDa bySDS-PAGE, and this discrepancy may be due to the acidic nature of the protein, which has a calculated isoelectric point of 5.3 (PMID: 12805216).

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