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RFC4 Monoclonal antibody

RFC4 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A6A11

Cat No : 68390-1-Ig

Print datasheet

Synonyms

A1, A1 37 kDa subunit, Activator 1 37 kDa subunit, Activator 1 subunit 4, Replication factor C subunit 4, RF C 37 kDa subunit, RFC37, RFC4

验证数据展示

  • WB analysis using 68390-1-Ig

    WB analysis using 68390-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68390-1-Ig (RFC4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

经过测试的应用

Positive WB detected inU2OS cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, 4T1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68390-1-Ig targets RFC4 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA
Tested Reactivity Human, mouse, rat
Immunogen RFC4 fusion protein Ag33295 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name replication factor C (activator 1) 4, 37kDa
Synonyms A1, A1 37 kDa subunit, Activator 1 37 kDa subunit, Activator 1 subunit 4, Replication factor C subunit 4, RF C 37 kDa subunit, RFC37, RFC4
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 37-40 kDa
GenBank Accession NumberBC017452
Gene Symbol RFC4
Gene ID (NCBI) 5984
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP35249
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RFC4 antibody 68390-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols