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验证数据展示

  • WB analysis using 29601-1-AP

    WB analysis using 29601-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29601-1-AP (RELB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of Raji using 29601-1-AP

    IP experiment of Raji using 29601-1-AP

    IP result of anti-RELB (IP:29601-1-AP, 4ug; Detection:29601-1-AP 1:1000) with Raji cells lysate 2840 ug.

  • IHC staining of human ovary cancer using 29601-1-AP

    IHC staining of human ovary cancer using 29601-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 29601-1-AP (RELB antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RELB antibody included

RELB Polyclonal antibody

RELB Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 29601-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, ELISA

别名

Transcription factor RelB, I-Rel

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inRaji cells, Ramos cells
Positive IP detected inRaji cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy RELB Ready-To-Use IHC Kit. RELB primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29601-1-AP targets RELB in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag30334

Product name: Recombinant human RELB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 375-446 aa of BC028013

Sequence: VTVNVFLQRLTDGVCSEPLPFTYLPRDHDSYGVDKKRKRGMPDVLGELNSSDPHGIESKRRKKKPAILDHFL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 v-rel reticuloendotheliosis viral oncogene homolog B
别名 Transcription factor RelB, I-Rel
计算分子量 62 kDa
观测分子量 62-65 kDa
GenBank蛋白编号BC028013
基因名称 RELB
Gene ID (NCBI) 5971
RRIDAB_2918330
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ01201
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RELB is one of the more unusual members of the NF-κB family, it is best known for its roles in lymphoid development, DC biology, and noncanonical signaling. RELB is capable of direct binding to the AhR that supports the xenobiotic-detoxifying pathway. RELB can regulate the circadian rhythm by directly binding to the BMAL partner of CLOCK.

实验方案

Product Specific Protocols
IHC protocol for RELB antibody 29601-1-APDownload protocol
IP protocol for RELB antibody 29601-1-APDownload protocol
WB protocol for RELB antibody 29601-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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