验证数据展示
经过测试的应用
| Positive WB detected in | Daudi cells, HEK-293 cells, Jurkat cells, MOLT-4 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
67747-1-Ig targets REL in WB, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human |
| 免疫原 |
CatNo: Ag26803 Product name: Recombinant human REL protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 279-471 aa of NM_001291746 Sequence: MYLPDEKDTYGNKAKKQKTTLLFQKLCQDHVETGFRHVDQDGLELLTSGDPPTLASQSAGITVNFPERPRPGLLGSIGEGRYFKKEPNLFSHDAVVREMPTGVSSQAESYYPSPGPISSGLSHHASMAPLPSSSWSSVAHPTPRSGNTNPLSSFSTRTLPSNSQGIPPFLRIPVGNDLNASNACIYNNADDIVG 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | v-rel reticuloendotheliosis viral oncogene homolog (avian) |
| 别名 | C Rel, Proto oncogene c Rel, REL |
| 计算分子量 | 69 kDa |
| 观测分子量 | 68 kDa, 80 kDa |
| GenBank蛋白编号 | NM_001291746 |
| 基因名称 | REL |
| Gene ID (NCBI) | 5966 |
| RRID | AB_2918516 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q04864 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
REL, also known as, Proto-oncogene c-Rel, is a 619 amino acid protein, which may play a role in differentiation and lymphopoiesis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The NF-kappa-B heterodimer RELA/p65-c-Rel is a transcriptional activator. The full-length 80-kDa c-Rel was identified (PMID: 9857058).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for REL antibody 67747-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
