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REL Monoclonal antibody

REL Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F6A4

Cat No : 67747-1-Ig

Print datasheet

Synonyms

C Rel, Proto oncogene c Rel, REL

验证数据展示

  • WB analysis using 67747-1-Ig

    WB analysis using 67747-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67747-1-Ig (REL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

经过测试的应用

Positive WB detected inDaudi cells, HEK-293 cells, Jurkat cells, MOLT-4 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67747-1-Ig targets REL in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA
Tested Reactivity Human
Immunogen REL fusion protein Ag26803 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name v-rel reticuloendotheliosis viral oncogene homolog (avian)
Synonyms C Rel, Proto oncogene c Rel, REL
Calculated Molecular Weight 69 kDa
Observed Molecular Weight68 kDa, 80 kDa
GenBank Accession NumberNM_001291746
Gene Symbol REL
Gene ID (NCBI) 5966
RRIDAB_2918516
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ04864
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

REL, also known as, Proto-oncogene c-Rel, is a 619 amino acid protein, which may play a role in differentiation and lymphopoiesis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The NF-kappa-B heterodimer RELA/p65-c-Rel is a transcriptional activator. The full-length 80-kDa c-Rel was identified (PMID: 9857058).

实验方案

Product Specific Protocols
WB protocol for REL antibody 67747-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols