验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, L02 cells, MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
23140-1-AP targets REEP3 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag19400 Product name: Recombinant human REEP3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 73-255 aa of BC068557 Sequence: VIWLLSPYTKGASLIYRKFLHPLLSSKEREIDDYIVQAKERGYETMVNFGRQGLNLAATAAVTAAVKSQGAITERLRSFSMHDLTTIQGDEPVGQRPYQPLPEAKKKSKPAPSESAGYGIPLKDGDEKTDEEAEGPYSDNEMLTHKGLRRSQSMKSVKTTKGRKEVRYGSLKYKVKKRPQVYF 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | receptor accessory protein 3 |
| 别名 | C10orf74, receptor accessory protein 3 |
| 计算分子量 | 255 aa, 29 kDa |
| 观测分子量 | 29 kDa |
| GenBank蛋白编号 | BC068557 |
| 基因名称 | REEP3 |
| Gene ID (NCBI) | 221035 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6NUK4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
REEP3 (Receptor Expression-Enhancing Protein 3) is a member of the REEP family of proteins, a transmembrane protein that interacts with odorant receptor proteins and may enhance the odorant receptor responses to odorants. It primarily regulates the tubular morphology of the endoplasmic reticulum by promoting high membrane curvature through its reticular homology domains. During mitosis, REEP3 binds to microtubules, separating the endoplasmic reticulum from chromosomes to ensure proper nuclear membrane remodeling and cell division.(PMID: 23911198; 30995177)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for REEP3 antibody 23140-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
