Various lysates were subjected to SDS PAGE followed by western blot with 15684-1-AP (REEP2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15684-1-PBS in a different storage buffer formulation.

1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug REEP2 Polyclonal antibody (15684-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 15684-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15684-1-AP (REEP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15684-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15684-1-AP (REEP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15684-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 15684-1-AP (REEP2 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15684-1-PBS in a different storage buffer formulation.

IP result of anti-REEP2 (IP:15684-1-AP, 4ug; Detection:15684-1-AP 1:5000) with mouse brain tissue lysate 1680 ug. This data was developed using the same antibody clone with 15684-1-PBS in a different storage buffer formulation.