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验证数据展示

  • IF Staining of HeLa using CL488-17008

    IF Staining of HeLa using CL488-17008

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 RECQL4 antibody (CL488-17008) at dilution of 1:200, CL594-Phalloidin (red).

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  • KD/KO验证

CoraLite® Plus 488-conjugated RECQL4 Polyclonal antibody

RECQL4 Polyclonal Antibody for IF/ICC
Cat No. CL488-17008

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

别名

ATP dependent DNA helicase Q4, DNA helicase, RecQ like type 4, RecQ protein like 4, RECQ4, RECQL4, RTS

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RECQL4 Ready-To-Use IHC Kit. RECQL4 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 17008-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-17008 targets RECQL4 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原

CatNo: Ag10707

Product name: Recombinant human RECQL4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 297-652 aa of BC013277

Sequence: CTCTCTRPPSEQEGAVGGERPVPKYPPQEAEQLSHQAAPGPRRVCMGHERALPIQLTVQALDMPEEAIETLLCYLELHPHHWLELLATTYTHCRLNCPGGPAQLQALAHRCPPLAVCLAQQLPEDPGQGSSSVEFDMVKLVDSMGWELASVRRALCQLQWDHEPRTGVRRGTGVLVEFSELAFHLRSPGDLTAEEKDQICDFLYGRVQARERQALARLRRTFQAFHSVAFPSCGPCLEQQDEERSTRLKDLLGRYFEEEEGQEPGGMEDAQGPEPGQARLQDWEDQVRCDIRQFLSLRPEEKFSSRAVARIFHGIGSPCYPAQVYGQDRRFWRKYLHLSFHALVGLATEELLQVAR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RecQ protein-like 4
别名 ATP dependent DNA helicase Q4, DNA helicase, RecQ like type 4, RecQ protein like 4, RECQ4, RECQL4, RTS
计算分子量 1208 aa, 133 kDa
观测分子量 145-150 kDa
GenBank蛋白编号BC013277
基因名称 RECQL4
Gene ID (NCBI) 9401
RRIDAB_3672665
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO94761
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RECQ protein-like 4(RECOL4), which has helicase domains contain 7 consensus motifs in the middle, is a gene responsible for Werner syndorme and Bloom syndrome. It forms a stable complex with UBR1 adn UBR2, which has a DNA-stimulated ATPase activity. RECOL4 may involve in repairment of DNA damage by interaction with APE1 and FEN1, key participants in base excision repair. This is a rabbit polyclonal antibody raised against part of C-terminal of RECQ4 of human origin. The calculated molecular weight of EIF3B is 133 kDa, but the modified EIF3B protein is about 145-150 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 RECQL4 antibody CL488-17008Download protocol
Standard Protocols
Click here to view our Standard Protocols
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