验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, A431 cells |
| Positive IF/ICC detected in | A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
86529-2-RR targets RECQL4 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag10707 Product name: Recombinant human RECQL4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 297-652 aa of BC013277 Sequence: CTCTCTRPPSEQEGAVGGERPVPKYPPQEAEQLSHQAAPGPRRVCMGHERALPIQLTVQALDMPEEAIETLLCYLELHPHHWLELLATTYTHCRLNCPGGPAQLQALAHRCPPLAVCLAQQLPEDPGQGSSSVEFDMVKLVDSMGWELASVRRALCQLQWDHEPRTGVRRGTGVLVEFSELAFHLRSPGDLTAEEKDQICDFLYGRVQARERQALARLRRTFQAFHSVAFPSCGPCLEQQDEERSTRLKDLLGRYFEEEEGQEPGGMEDAQGPEPGQARLQDWEDQVRCDIRQFLSLRPEEKFSSRAVARIFHGIGSPCYPAQVYGQDRRFWRKYLHLSFHALVGLATEELLQVAR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | RecQ protein-like 4 |
| 别名 | ATP dependent DNA helicase Q4, ATP-dependent DNA helicase Q4, DNA 3'-5' helicase RecQ4, DNA helicase, RecQ-like type 4, EC:5.6.2.4 |
| 计算分子量 | 1208 aa, 133 kDa |
| 观测分子量 | 140-150 kDa |
| GenBank蛋白编号 | BC013277 |
| 基因名称 | RECQL4 |
| Gene ID (NCBI) | 9401 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O94761 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RECQ protein-like 4(RECOL4), which has helicase domains contain 7 consensus motifs in the middle, is a gene responsible for Werner syndorme and Bloom syndrome. It forms a stable complex with UBR1 adn UBR2, which has a DNA-stimulated ATPase activity. RECOL4 may involve in repairment of DNA damage by interaction with APE1 and FEN1, key participants in base excision repair. The calculated molecular weight of EIF3B is 133 kDa, but the modified EIF3B protein is about 145-150 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for RECQL4 antibody 86529-2-RR | Download protocol |
| WB protocol for RECQL4 antibody 86529-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
