×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 86529-2-RR (same clone as 86529-2-PBS)

    WB analysis using 86529-2-RR (same clone as 86529-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86529-2-RR (RECQL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86529-2-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 86529-2-RR (same clone as 86529-2-PBS)

    IF Staining of A431 using 86529-2-RR (same clone as 86529-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using RECQL4 antibody (86529-2-RR, Clone: 251312D3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86529-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86529-2-RR

    Affinity and Kinetic Characterization of 86529-2-RR

    Biolayer interferometry (BLl) kinetic assays of 86529-2-RR against Human RECQL4 were performed. The affinity constant is below 1 pM.

RECQL4 Recombinant monoclonal antibody, PBS Only

RECQL4 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86529-2-PBS

产品说明书

CloneNo. 251312D3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

ATP dependent DNA helicase Q4, ATP-dependent DNA helicase Q4, DNA 3'-5' helicase RecQ4, DNA helicase, RecQ-like type 4, EC:5.6.2.4

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy RECQL4 Ready-To-Use IHC Kit. RECQL4 primary antibody included.

产品信息

86529-2-PBS targets RECQL4 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag10707

Product name: Recombinant human RECQL4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 297-652 aa of BC013277

Sequence: CTCTCTRPPSEQEGAVGGERPVPKYPPQEAEQLSHQAAPGPRRVCMGHERALPIQLTVQALDMPEEAIETLLCYLELHPHHWLELLATTYTHCRLNCPGGPAQLQALAHRCPPLAVCLAQQLPEDPGQGSSSVEFDMVKLVDSMGWELASVRRALCQLQWDHEPRTGVRRGTGVLVEFSELAFHLRSPGDLTAEEKDQICDFLYGRVQARERQALARLRRTFQAFHSVAFPSCGPCLEQQDEERSTRLKDLLGRYFEEEEGQEPGGMEDAQGPEPGQARLQDWEDQVRCDIRQFLSLRPEEKFSSRAVARIFHGIGSPCYPAQVYGQDRRFWRKYLHLSFHALVGLATEELLQVAR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RecQ protein-like 4
别名 ATP dependent DNA helicase Q4, ATP-dependent DNA helicase Q4, DNA 3'-5' helicase RecQ4, DNA helicase, RecQ-like type 4, EC:5.6.2.4
计算分子量 1208 aa, 133 kDa
观测分子量140-150 kDa
GenBank蛋白编号BC013277
基因名称 RECQL4
Gene ID (NCBI) 9401
RRIDAB_3744946
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO94761
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RECQ protein-like 4(RECOL4), which has helicase domains contain 7 consensus motifs in the middle, is a gene responsible for Werner syndorme and Bloom syndrome. It forms a stable complex with UBR1 adn UBR2, which has a DNA-stimulated ATPase activity. RECOL4 may involve in repairment of DNA damage by interaction with APE1 and FEN1, key participants in base excision repair. The calculated molecular weight of EIF3B is 133 kDa, but the modified EIF3B protein is about 145-150 kDa.

||
New chat

Able™

正在加载，请稍候...