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验证数据展示

  • WB analysis using 86529-2-RR (same clone as 86529-2-PBS)

    WB analysis using 86529-2-RR (same clone as 86529-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86529-2-RR (RECQL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86529-2-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 86529-2-RR (same clone as 86529-2-PBS)

    IF Staining of A431 using 86529-2-RR (same clone as 86529-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using RECQL4 antibody (86529-2-RR, Clone: 251312D3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86529-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86529-2-RR

    Affinity and Kinetic Characterization of 86529-2-RR

    Biolayer interferometry (BLl) kinetic assays of 86529-2-RR against Human RECQL4 were performed. The affinity constant is below 1 pM.

RECQL4 Recombinant monoclonal antibody, PBS Only

RECQL4 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86529-2-PBS

产品说明书

CloneNo. 251312D3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

ATP dependent DNA helicase Q4, ATP-dependent DNA helicase Q4, DNA 3'-5' helicase RecQ4, DNA helicase, RecQ-like type 4, EC:5.6.2.4

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy RECQL4 Ready-To-Use IHC Kit. RECQL4 primary antibody included.

产品信息

86529-2-PBS targets RECQL4 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag10707

Product name: Recombinant human RECQL4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 297-652 aa of BC013277

Sequence: CTCTCTRPPSEQEGAVGGERPVPKYPPQEAEQLSHQAAPGPRRVCMGHERALPIQLTVQALDMPEEAIETLLCYLELHPHHWLELLATTYTHCRLNCPGGPAQLQALAHRCPPLAVCLAQQLPEDPGQGSSSVEFDMVKLVDSMGWELASVRRALCQLQWDHEPRTGVRRGTGVLVEFSELAFHLRSPGDLTAEEKDQICDFLYGRVQARERQALARLRRTFQAFHSVAFPSCGPCLEQQDEERSTRLKDLLGRYFEEEEGQEPGGMEDAQGPEPGQARLQDWEDQVRCDIRQFLSLRPEEKFSSRAVARIFHGIGSPCYPAQVYGQDRRFWRKYLHLSFHALVGLATEELLQVAR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RecQ protein-like 4
别名 ATP dependent DNA helicase Q4, ATP-dependent DNA helicase Q4, DNA 3'-5' helicase RecQ4, DNA helicase, RecQ-like type 4, EC:5.6.2.4
计算分子量 1208 aa, 133 kDa
观测分子量140-150 kDa
GenBank蛋白编号BC013277
基因名称 RECQL4
Gene ID (NCBI) 9401
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO94761
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RECQ protein-like 4(RECOL4), which has helicase domains contain 7 consensus motifs in the middle, is a gene responsible for Werner syndorme and Bloom syndrome. It forms a stable complex with UBR1 adn UBR2, which has a DNA-stimulated ATPase activity. RECOL4 may involve in repairment of DNA damage by interaction with APE1 and FEN1, key participants in base excision repair. This is a rabbit polyclonal antibody raised against part of C-terminal of RECQ4 of human origin. The calculated molecular weight of EIF3B is 133 kDa, but the modified EIF3B protein is about 145-150 kDa.

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