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RDX Polyclonal antibody

RDX Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 26105-1-AP

Print datasheet

Synonyms

DFNB24, radixin, RDX

验证数据展示

  • WB analysis using 26105-1-AP

    WB analysis using 26105-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26105-1-AP (RDX antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse liver using 26105-1-AP

    WB analysis of mouse liver using 26105-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 26105-1-AP (RDX Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 26105-1-AP

    IF Staining of HeLa using 26105-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 26105-1-AP (RDX antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inCOS-7 cells, HeLa cells, Jurkat cells, mouse liver tissue, C6 cells, mouse brain tissue, mouse lung tissue, rat brain tissue
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26105-1-AP targets RDX in WB, IF, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity human, mouse, rat, monkey
Immunogen RDX fusion protein Ag23532 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name radixin
Synonyms DFNB24, radixin, RDX
Calculated Molecular Weight 583 aa, 68 kDa
Observed Molecular Weight 70-75 kDa
GenBank Accession NumberBC047109
Gene Symbol RDX
Gene ID (NCBI) 5962
RRIDAB_2880380
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35241
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RDX antibody 26105-1-APDownload protocol
IF protocol for RDX antibody 26105-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols