验证数据展示
经过测试的应用
| Positive WB detected in | COS-7 cells, HeLa cells, Jurkat cells, mouse liver tissue, C6 cells, mouse brain tissue, mouse lung tissue, rat brain tissue |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
26105-1-AP targets Radixin in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | human, mouse, rat, monkey |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag23532 Product name: Recombinant human RDX protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 384-455 aa of BC047109 Sequence: EEAERLEKERRAAEEAKSAIAKQAADQMKNQEQLAAELAEFTAKIALLEEAKKKKEEEATEWQHKAFAAQED 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | radixin |
| 别名 | RDX |
| 计算分子量 | 583 aa, 68 kDa |
| 观测分子量 | 70-75 kDa |
| GenBank蛋白编号 | BC047109 |
| 基因名称 | RDX |
| Gene ID (NCBI) | 5962 |
| RRID | AB_2880380 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P35241 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Radixin belongs to the ezrin-radixin-moesin (ERM) family of proteins which act as cross-linkers between membrane and actin cytoskeleton. ERM proteins provide structural links to strengthen the cell cortex and facilitate several key cellular processes, including membrane dynamics, substrate adhesion, cell survival, cell adhesion, and motility. The function of ERM proteins is highly reliant on phosphorylation induced conformational changes in response to chemokine and antigen stimulation.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Radixin antibody 26105-1-AP | Download protocol |
| WB protocol for Radixin antibody 26105-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
