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验证数据展示

  • WB analysis using 26105-1-AP

    WB analysis using 26105-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26105-1-AP (RDX antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse liver using 26105-1-AP

    WB analysis of mouse liver using 26105-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 26105-1-AP (RDX Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 26105-1-AP

    IF Staining of HeLa using 26105-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 26105-1-AP (RDX antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

RDX Polyclonal antibody

RDX Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 26105-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, monkey

应用

WB, IF/ICC, ELISA

别名

DFNB24, radixin, RDX

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCOS-7 cells, HeLa cells, Jurkat cells, mouse liver tissue, C6 cells, mouse brain tissue, mouse lung tissue, rat brain tissue
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy Radixin/RDX Ready-To-Use IHC Kit. Radixin/RDX primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26105-1-AP targets RDX in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat, monkey
免疫原 RDX fusion protein Ag23532 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 radixin
别名 DFNB24, radixin, RDX
计算分子量 583 aa, 68 kDa
观测分子量 70-75 kDa
GenBank蛋白编号BC047109
基因名称 RDX
Gene ID (NCBI) 5962
RRIDAB_2880380
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP35241
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RDX antibody 26105-1-APDownload protocol
IF protocol for RDX antibody 26105-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols