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验证数据展示

  • WB analysis of PC-3 using 20156-1-AP (same clone as 20156-1-PBS)

    WB analysis of PC-3 using 20156-1-AP (same clone as 20156-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 20156-1-AP (RDM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20156-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung using 20156-1-AP (same clone as 20156-1-PBS)

    IHC staining of human lung using 20156-1-AP (same clone as 20156-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 20156-1-AP (RDM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20156-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung using 20156-1-AP (same clone as 20156-1-PBS)

    IHC staining of human lung using 20156-1-AP (same clone as 20156-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 20156-1-AP (RDM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20156-1-PBS in a different storage buffer formulation.

  • IF Staining of PC-3 using 20156-1-AP (same clone as 20156-1-PBS)

    IF Staining of PC-3 using 20156-1-AP (same clone as 20156-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed PC-3 cells using 20156-1-AP (RDM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20156-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

RDM1 Polyclonal antibody, PBS Only

RDM1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 20156-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

20156-1-PBS targets RDM1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag14077

Product name: Recombinant human RDM1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-253 aa of BC038301

Sequence: QHSLFTAFSQFGLLYSVRVFPNAAVAHPGFYAVIKFYSARAAHRAQKACDRKQLFQKSPVKVRLGTRHKAVQHQALALNSSKCQELANYCFGFNGCSKRIIKLQELSDLEERENEDSMVPLPKQSLKFFCALEVVLPSCDCRSPGIGLVEEPMDKVEEGPLSFLMKRKTAQKLAIQKALSDAFQKLLIVVLESGKIAVEYRPSEDIVGVRCEEELHGLIQVPCSPWKQYGQEEEGYLSDFSLEEEEFRLPELD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAD52 motif 1
别名
计算分子量 284 aa, 32 kDa
观测分子量 32 kDa
GenBank蛋白编号BC038301
基因名称 RDM1
Gene ID (NCBI) 201299
RRIDAB_10666433
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NG50
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RDM1, also named as RAD52B, encodes a RNA recognition motif (RRM)-containing protein confered resistance to the antitumor agent cisplatin. The expression of RDM1 is closely related to the cell proliferation and apoptosis of thyroid carcinoma cells and RDM1 may be a potential marker of lung adenocarcinoma. RDM1 has many isoforms (8 kDa, 12-19 kDa and 25-32 kDa) with long or short N-terminus and a great diversity in the exonic composition of C-terminus generated by alternative pre-mRNA splicing and differential usage of two translational start sites. RDM1 protein can be expressed differently in cytoplasm or nuclear/nucleolar in the absence of stress, proteotoxic stress and mild heat shock, suggesting functional differences among the RDM1 proteins. ( PMID: 28939762, 17905820, 28939762, 30069034)

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