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RDM1 Polyclonal antibody

RDM1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 20156-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA and More (1)

RAD52 homolog B, RAD52 motif 1, RAD52B, RDM1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inPC-3 cells, human placenta tissue, MCF-7 cells
Positive IHC detected inhuman lung tissue, human brain tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20156-1-AP targets RDM1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF, IHC
经测试反应性 human
文献引用反应性human
免疫原 RDM1 fusion protein Ag14077 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAD52 motif 1
别名 RAD52 homolog B, RAD52 motif 1, RAD52B, RDM1
计算分子量 284 aa, 32 kDa
观测分子量 32 kDa
GenBank蛋白编号BC038301
基因名称 RDM1
Gene ID (NCBI) 201299
RRIDAB_10666433
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NG50
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RDM1, also named as RAD52B, encodes a RNA recognition motif (RRM)-containing protein confered resistance to the antitumor agent cisplatin. The expression of RDM1 is closely related to the cell proliferation and apoptosis of thyroid carcinoma cells and RDM1 may be a potential marker of lung adenocarcinoma. RDM1 has many isoforms (8 kDa, 12-19 kDa and 25-32 kDa) with long or short N-terminus and a great diversity in the exonic composition of C-terminus generated by alternative pre-mRNA splicing and differential usage of two translational start sites. RDM1 protein can be expressed differently in cytoplasm or nuclear/nucleolar in the absence of stress, proteotoxic stress and mild heat shock, suggesting functional differences among the RDM1 proteins. ( PMID: 28939762, 17905820, 28939762, 30069034)

实验方案

Product Specific Protocols
WB protocol for RDM1 antibody 20156-1-APDownload protocol
IHC protocol for RDM1 antibody 20156-1-APDownload protocol
IF protocol for RDM1 antibody 20156-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC,IF

Mol Oncol

Loss of RDM1 enhances hepatocellular carcinoma progression via p53 and Ras/Raf/ERK pathways.

Authors - Shi-Lu Chen
  • KD Validated
humanWB

J Cell Mol Med

RDM1 promotes critical processes in breast cancer tumorigenesis.

Authors - Yajun Chen
  • KD Validated
humanWB

Front Cell Dev Biol

A Novel Signature Constructed by RNA-Binding Protein Coding Genes to Improve Overall Survival Prediction of Glioma Patients.

Authors - Zewei Tu
humanWB,IHC

Artif Cells Nanomed Biotechnol

RDM1 plays an oncogenic role in human ovarian carcinoma cells.

Authors - Lu Tong
  • KD Validated
humanWB,IHC

Sci Rep

RDM1 plays an oncogenic role in human lung adenocarcinoma cells.

Authors - Lu Tong
  • KD Validated
humanWB,IHC

FEBS Open Bio

RDM1 promotes neuroblastoma growth through the RAS-Raf-MEK-ERK pathway.

Authors - Guojin Xie
  • KD Validated
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