验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, mouse brain tissue, mouse eye tissue, mouse placenta tissue, mouse kidney tissue, rat kidney tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse testis tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | hela cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10705-1-AP targets NELFE in WB, IHC, IF/ICC, FC (Intra), IP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, ChIP, RIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag1186 Product name: Recombinant human RDBP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-333 aa of BC011600 Sequence: MLVIPPGLSEEEEALQKKFNKLKKKKKALLALKKQSSSSTTSQGGVKRSLSEQPVMDTATATEQAKQLVKSGAISAIKAETKNSGFKRSRTLEGKLKDPEKGPVPTFQPFQRSISADDDLQESSRRPQRKSLYESDRLRELGPDGEEAEGPGAGDGPPRSFDWGYEERSGAHSSASPPRSRSRDRSHERNRDRDREGPFRRSDSFPERRAPRKGNTLYVYGEDMTPTLLRGAFSPFGNIIDLSMDPPRNCAFVTYEKMESADQAVAELNGTQVESVQLKVNIARKQPMLDAATGKSVWGSLAVQNSPKGCHRDKRTQIVYSDDVYKENLVDGF 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RD RNA binding protein |
| 别名 | NELF E, NELF-E, RD, RDBP, RNA-binding protein RD |
| 计算分子量 | 37 kDa, 43 kDa |
| 观测分子量 | 43 kDa |
| GenBank蛋白编号 | BC011600 |
| 基因名称 | NELFE |
| Gene ID (NCBI) | 7936 |
| RRID | AB_513966 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P18615 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RDBP, also named NELF-E and RD, belongs to the RRM NELF-E family. It is an essential component of the NELF complex which is a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, a complex acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. RDBP was recently found implicated in RNA polymerase II pausing by association with Hsp90. Catalog# 10705-1-AP is a widely used rabbit polyclonal antibody obtained by immunizing animals with full-length RDBP of human origin.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NELFE antibody 10705-1-AP | Download protocol |
| IHC protocol for NELFE antibody 10705-1-AP | Download protocol |
| IF protocol for NELFE antibody 10705-1-AP | Download protocol |
| IP protocol for NELFE antibody 10705-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Hsp90 globally targets paused RNA polymerase to regulate gene expression in response to environmental stimuli. | ||
Nat Commun Negative elongation factor complex enables macrophage inflammatory responses by controlling anti-inflammatory gene expression. | ||
Nat Commun RNA polymerase II pausing factor NELF in CD8+ T cells promotes antitumor immunity. | ||
Dev Cell Negative elongation factor regulates muscle progenitor expansion for efficient myofiber repair and stem cell pool repopulation. | ||
Proc Natl Acad Sci U S A Immediate mediators of the inflammatory response are poised for gene activation through RNA polymerase II stalling. |
