• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

NELFE Polyclonal antibody

NELFE Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IF/ICC, IHC, IP, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 10705-1-AP

Print datasheet

Synonyms

D6S45, Negative elongation factor E, NELF E, NELFE, RD, RD RNA binding protein, RDBP, RDP, RNA binding protein RD

验证数据展示

  • WB analysis of HEK-293 using 10705-1-AP

    WB analysis of HEK-293 using 10705-1-AP

    WB result of NELFE antibody (10705-1-AP; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NELFE transfected HEK-293 cells.

  • WB analysis using 10705-1-AP

    WB analysis using 10705-1-AP

    Various lyastes were subjected to SDS PAGE followed by western blot with 10705-1-AP (NELFE antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 10705-1-AP

    IHC staining of human prostate cancer using 10705-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10705-1-AP (NELFE antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with NELFE antibody included
  • IP experiment of mouse brain using 10705-1-AP

    IP experiment of mouse brain using 10705-1-AP

    IP result of anti-NELFE (IP:10705-1-AP, 4ug; Detection:10705-1-AP 1:800) with mouse brain tissue lysate 4680ug.

  • IF Staining of HeLa using 10705-1-AP

    IF Staining of HeLa using 10705-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 10705-1-AP (NELFE antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of hela using 10705-1-AP

    FC experiment of hela using 10705-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human NELFE (10705-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive WB detected inHEK-293 cells, mouse brain tissue, mouse eye tissue, mouse placenta tissue, rat brain tissue, HeLa cells, mouse kidney tissue, rat kidney tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy NELFE Ready-To-Use IHC Kit. NELFE primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10705-1-AP targets NELFE in WB, IP, IF, FC, RIP, IHC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB, IF, RIP, IHC, ChIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen NELFE fusion protein Ag1186 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RD RNA binding protein
Synonyms D6S45, Negative elongation factor E, NELF E, NELFE, RD, RD RNA binding protein, RDBP, RDP, RNA binding protein RD
Calculated Molecular Weight 37 kDa, 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC011600
Gene Symbol RDBP
Gene ID (NCBI) 7936
RRIDAB_513966
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP18615
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RDBP, also named NELF-E and RD, belongs to the RRM NELF-E family. It is an essential component of the NELF complex which is a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, a complex acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. RDBP was recently found implicated in RNA polymerase II pausing by association with Hsp90. Catalog# 10705-1-AP is a widely used rabbit polyclonal antibody obtained by immunizing animals with full-length RDBP of human origin.

实验方案

Product Specific Protocols
WB protocol for NELFE antibody 10705-1-APDownload protocol
IHC protocol for NELFE antibody 10705-1-APDownload protocol
IF protocol for NELFE antibody 10705-1-APDownload protocol
IP protocol for NELFE antibody 10705-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Nature

The landscape of RNA Pol II binding reveals a stepwise transition during ZGA.

Authors - Bofeng Liu
View Article
human, mouseWB

Cell

Hsp90 globally targets paused RNA polymerase to regulate gene expression in response to environmental stimuli.

Authors - Sawarkar Ritwick R
View Article
mouseWB,chIP

Nat Commun

Negative elongation factor complex enables macrophage inflammatory responses by controlling anti-inflammatory gene expression.

Authors - Li Yu
View Article
mouseWB

Nat Commun

RNA polymerase II pausing factor NELF in CD8+ T cells promotes antitumor immunity.

Authors - Bogang Wu
View Article
mouseWB

Dev Cell

Negative elongation factor regulates muscle progenitor expansion for efficient myofiber repair and stem cell pool repopulation.

Authors - Daniel C L Robinson
View Article
mouseWB,IHC

Proc Natl Acad Sci U S A

Immediate mediators of the inflammatory response are poised for gene activation through RNA polymerase II stalling.

Authors - Adelman Karen K
View Article