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验证数据展示

  • WB analysis of HEK-293 using 67856-1-Ig (same clone as 67856-1-PBS)

    WB analysis of HEK-293 using 67856-1-Ig (same clone as 67856-1-PBS)

    WB result of RCHY1 antibody (67856-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RCHY1 transfected HEK-293 cells. This data was developed using the same antibody clone with 67856-1-PBS in a different storage buffer formulation.

  • WB analysis using 67856-1-Ig (same clone as 67856-1-PBS)

    WB analysis using 67856-1-Ig (same clone as 67856-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67856-1-Ig (RCHY1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67856-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67856-1-Ig (same clone as 67856-1-PBS)

    IHC staining of human breast cancer using 67856-1-Ig (same clone as 67856-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67856-1-Ig (RCHY1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67856-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RCHY1/Pirh2 antibody included
  • IHC staining of human breast cancer using 67856-1-Ig (same clone as 67856-1-PBS)

    IHC staining of human breast cancer using 67856-1-Ig (same clone as 67856-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67856-1-Ig (RCHY1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67856-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RCHY1/Pirh2 antibody included
  • IF Staining of A549 using 67856-1-Ig (same clone as 67856-1-PBS)

    IF Staining of A549 using 67856-1-Ig (same clone as 67856-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using RCHY1 antibody (67856-1-Ig, Clone: 2C11C11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67856-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67856-1-Ig (same clone as 67856-1-PBS)

    IF Staining of HeLa using 67856-1-Ig (same clone as 67856-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RCHY1 antibody (67856-1-Ig, Clone: 2C11C11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67856-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

RCHY1 Monoclonal antibody, PBS Only

RCHY1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67856-1-PBS

产品说明书

CloneNo. 2C11C11

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

PIRH2, Androgen receptor N-terminal-interacting protein, ARNIP, CHIMP, CH-rich-interacting match with PLAG1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy RCHY1/Pirh2 Ready-To-Use IHC Kit. RCHY1/Pirh2 primary antibody included.

产品信息

67856-1-PBS targets RCHY1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag4942

Product name: Recombinant human RCHY1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-261 aa of BC047393

Sequence: MAATAREDGASGQERGQRGCEHYDRGCLLKAPCCDKLYTCRLCHDNNEDHQLDRFKVKEVQCINCEKIQHAQQTCEECSTLFGEYYCDICHLFDKDKKQYHCENCGICRIGPKEDFFHCLKCNLCLAMNLQGRHKCIENVSQQNCPICLEDIHTSRVVAHVLPCGHLLHRTCYEEMLKEGYRCPLCMHSALDMTRYWRQLDDEVAQTPMPSEYQNMTVDILCNDCNGRSTVQFHILGMKCKICESYNTAQAGGRRISLDQQ

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 ring finger and CHY zinc finger domain containing 1
别名 PIRH2, Androgen receptor N-terminal-interacting protein, ARNIP, CHIMP, CH-rich-interacting match with PLAG1
计算分子量 261 aa, 30 kDa
观测分子量 45 KDa, 30 kDa
GenBank蛋白编号BC047393
基因名称 RCHY1
Gene ID (NCBI) 25898
RRIDAB_2918614
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ96PM5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Pirh2 is also named as RCHY1, ARNIP, CHIMP, PIRH2, RNF199, ZNF363 and has p53-induced ubiquitin-protein ligase activity, promoting p53 degradation. Pirh2 monoubiquitinates the translesion DNA polymerase POLH and contributes to the regulation of the cell cycle progression. This protein has 8 isoforms produced by alternative splicing and can exsit as a monomer or homodimer.

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