验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, LNCaP cells, Jurkat cells, K-562 cells, Daudi cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, A549 cells |
Planning an IHC experiment? We recommend our IHCeasy RCHY1/Pirh2 Ready-To-Use IHC Kit. RCHY1/Pirh2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
产品信息
67856-1-Ig targets RCHY1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | RCHY1 fusion protein Ag4942 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ring finger and CHY zinc finger domain containing 1 |
| 别名 | PIRH2, Androgen receptor N-terminal-interacting protein, ARNIP, CHIMP, CH-rich-interacting match with PLAG1 |
| 计算分子量 | 261 aa, 30 kDa |
| 观测分子量 | 45 KDa, 30 kDa |
| GenBank蛋白编号 | BC047393 |
| 基因名称 | RCHY1 |
| Gene ID (NCBI) | 25898 |
| RRID | AB_2918614 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q96PM5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Pirh2 is also named as RCHY1, ARNIP, CHIMP, PIRH2, RNF199, ZNF363 and has p53-induced ubiquitin-protein ligase activity, promoting p53 degradation. Pirh2 monoubiquitinates the translesion DNA polymerase POLH and contributes to the regulation of the cell cycle progression. This protein has 8 isoforms produced by alternative splicing and can exsit as a monomer or homodimer.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RCHY1 antibody 67856-1-Ig | Download protocol |
| IHC protocol for RCHY1 antibody 67856-1-Ig | Download protocol |
| IF protocol for RCHY1 antibody 67856-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |