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RCC2 Polyclonal antibody

RCC2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 16755-1-AP

Print datasheet

Synonyms

KIAA1470, Protein RCC2, RCC1 like protein TD 60, RCC2, TD 60, TD60

验证数据展示

  • WB analysis using 16755-1-AP

    WB analysis using 16755-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16755-1-AP (RCC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 16755-1-AP

    IHC staining of human colon cancer using 16755-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16755-1-AP (RCC2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 16755-1-AP

    IHC staining of human colon cancer using 16755-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16755-1-AP (RCC2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293T cells, PC-3 cells, HeLa cells, HUVEC cells, MOLT-4 cells, NCI-H1299 cells
Positive IHC detected inhuman colon cancer tissue, human ovary tumor tissue, human stomach cancer tissue, mouse testis tissue, rat colon tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16755-1-AP targets RCC2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen RCC2 fusion protein Ag10271 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name regulator of chromosome condensation 2
Synonyms KIAA1470, Protein RCC2, RCC1 like protein TD 60, RCC2, TD 60, TD60
Calculated Molecular Weight 522 aa, 56 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC042141
Gene Symbol RCC2
Gene ID (NCBI) 55920
RRIDAB_2878311
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P258
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RCC2 (also known as TD60) is a guanine exchange factor that is active on RalA, a small GTPase. RCC2 and RalA are both essential for proper kinetochore-microtubule function in early mitosis. RCC2 has been shown to be a biomarker for colorectal cancer.

实验方案

Product Specific Protocols
WB protocol for RCC2 antibody 16755-1-APDownload protocol
IHC protocol for RCC2 antibody 16755-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Exp Mol Med

The m6A reader IGF2BP3 promotes acute myeloid leukemia progression by enhancing RCC2 stability.

Authors - Nan Zhang
View Article