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验证数据展示

  • WB analysis using 16755-1-AP

    WB analysis using 16755-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16755-1-AP (RCC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 16755-1-AP

    IHC staining of human colon cancer using 16755-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16755-1-AP (RCC2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RCC2 antibody included
  • IHC staining of human colon cancer using 16755-1-AP

    IHC staining of human colon cancer using 16755-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16755-1-AP (RCC2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RCC2 antibody included

RCC2 Polyclonal antibody

RCC2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16755-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

KIAA1470, Protein RCC2, RCC1 like protein TD 60, RCC2, TD 60, TD60

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, PC-3 cells, HeLa cells, HUVEC cells, MOLT-4 cells, NCI-H1299 cells
Positive IHC detected inhuman colon cancer tissue, human ovary tumor tissue, human stomach cancer tissue, mouse testis tissue, rat colon tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy RCC2 Ready-To-Use IHC Kit. RCC2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

16755-1-AP targets RCC2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 RCC2 fusion protein Ag10271 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 regulator of chromosome condensation 2
别名 KIAA1470, Protein RCC2, RCC1 like protein TD 60, RCC2, TD 60, TD60
计算分子量 522 aa, 56 kDa
观测分子量 56 kDa
GenBank蛋白编号BC042141
基因名称 RCC2
Gene ID (NCBI) 55920
RRIDAB_2878311
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9P258
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RCC2 (also known as TD60) is a guanine exchange factor that is active on RalA, a small GTPase. RCC2 and RalA are both essential for proper kinetochore-microtubule function in early mitosis. RCC2 has been shown to be a biomarker for colorectal cancer.

实验方案

Product Specific Protocols
WB protocol for RCC2 antibody 16755-1-APDownload protocol
IHC protocol for RCC2 antibody 16755-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Exp Mol Med

The m6A reader IGF2BP3 promotes acute myeloid leukemia progression by enhancing RCC2 stability.

Authors - Nan Zhang
View Article