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验证数据展示

  • WB analysis using 31234-1-AP (same clone as 31234-1-PBS)

    WB analysis using 31234-1-AP (same clone as 31234-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31234-1-AP (RC3H1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31234-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293T using 31234-1-AP (same clone as 31234-1-PBS)

    WB analysis of HEK-293T using 31234-1-AP (same clone as 31234-1-PBS)

    WB result of RC3H1 antibody (31234-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RC3H1 transfected HEK-293T cells. This data was developed using the same antibody clone with 31234-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse pancreas using 31234-1-AP (same clone as 31234-1-PBS)

    IHC staining of mouse pancreas using 31234-1-AP (same clone as 31234-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 31234-1-AP (RC3H1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31234-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 31234-1-AP (same clone as 31234-1-PBS)

    IHC staining of human ovary cancer using 31234-1-AP (same clone as 31234-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31234-1-AP (RC3H1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31234-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 31234-1-AP (same clone as 31234-1-PBS)

    IF Staining of HeLa using 31234-1-AP (same clone as 31234-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RC3H1 antibody (31234-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 31234-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

RC3H1 Polyclonal antibody, PBS Only

RC3H1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 31234-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

KIAA2025, RING finger protein 198, RNF198, ROQUIN

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

31234-1-PBS targets RC3H1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag35089

Product name: Recombinant human RC3H1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1000-1133 aa of NM_001300850

Sequence: NTLAGQSQPPPPPPPKWPGMISSEQLSLELHQVEREIGKRTRELSMENQCSLDMKSKLNTSKQAENGQPEPQNKVPAEDLTLTFSDVPNGSALTQENISLLSNKTSSLNLSEDPEGGGDNNDSQRSGVTPSSAP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ring finger and CCCH-type zinc finger domains 1
别名 KIAA2025, RING finger protein 198, RNF198, ROQUIN
计算分子量126 kDa
观测分子量130-150 kDa
GenBank蛋白编号NM_001300850
基因名称 RC3H1
Gene ID (NCBI) 149041
RRIDAB_3669912
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ5TC82
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Roquin-1 (Rc3h1) is an E3 ubiquitin ligase originally discovered in a mutational screen for genetic factors contributory to systemic lupus erythematosus-like symptoms in mice. A single base pair mutation in the Rc3h1 gene resulted in the manifestation of autoantibody production and sustained immunological inflammation characterized by excessive T follicular helper cell activation and formation of germinal centers. (PMID: 26673963)

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