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验证数据展示

  • IF Staining of HeLa using CL594-66716

    IF Staining of HeLa using CL594-66716

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®®594 RBX1 antibody (CL594-66716, Clone: 1F4D9 ) at dilution of 1:100. DAPI (blue).

CoraLite®594-conjugated RBX1 Monoclonal antibody

RBX1 Monoclonal Antibody for IF/ICC
Cat No. CL594-66716

产品说明书

CloneNo. 1F4D9

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat

应用

IF/ICC

别名

BA554C12.1, Protein ZYP, RBX1, Regulator of cullins 1, ring box 1, RING box protein 1, RING finger protein 75, RNF75, ROC1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RBX1 Ready-To-Use IHC Kit. RBX1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66716-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66716 targets RBX1 in IF/ICC applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, Mouse, Rat
免疫原

CatNo: Ag7005

Product name: Recombinant human RBX1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-108 aa of BC001466

Sequence: MAAAMDVDTPSGTNSGAGKKRFEVKKWNAVALWAWDIVVDNCAICRNHIMDLCIECQANQASATSEECTVAWGVCNHAFHFHCISRWLKTRQVCPLDNREWEFQKYGH

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ring-box 1
别名 BA554C12.1, Protein ZYP, RBX1, Regulator of cullins 1, ring box 1, RING box protein 1, RING finger protein 75, RNF75, ROC1
计算分子量 12 kDa
观测分子量 12-15 kDa
GenBank蛋白编号BC001466
基因名称 RBX1
Gene ID (NCBI) 9978
RRIDAB_2920026
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDP62877
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RBX1(RING-box protein 1) is also named as RNF75, ROC1 and is a requisite component of the multi- subunit SCF family E3s36-40. It mediates the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair.

实验方案

Product Specific Protocols
IF protocol for CL594 RBX1 antibody CL594-66716Download protocol
Standard Protocols
Click here to view our Standard Protocols