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验证数据展示

  • IHC staining of human liver cancer using 66716-1-Ig (same clone as 66716-1-PBS)

    IHC staining of human liver cancer using 66716-1-Ig (same clone as 66716-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66716-1-Ig (RBX1 antibody) at dilution of 1:5000 (under 10x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 66716-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RBX1 antibody included
  • IHC staining of human liver cancer using 66716-1-Ig (same clone as 66716-1-PBS)

    IHC staining of human liver cancer using 66716-1-Ig (same clone as 66716-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66716-1-Ig (RBX1 antibody) at dilution of 1:5000 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 66716-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RBX1 antibody included
  • WB analysis using 66716-1-Ig (same clone as 66716-1-PBS)

    WB analysis using 66716-1-Ig (same clone as 66716-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66716-1-Ig (RBX1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66716-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 66716-1-Ig (same clone as 66716-1-PBS)

    IF Staining of A431 using 66716-1-Ig (same clone as 66716-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using RBX1 antibody (66716-1-Ig, Clone: 1F4D9 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red). This data was developed using the same antibody clone with 66716-1-PBS in a different storage buffer formulation.

RBX1 Monoclonal antibody, PBS Only

RBX1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66716-1-PBS

产品说明书

CloneNo. 1F4D9

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Protein ZYP, EC:2.3.2.27, E3 ubiquitin-protein transferase RBX1, E3 ubiquitin-protein ligase RBX1, N-terminally processed, E3 ubiquitin-protein ligase RBX1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy RBX1 Ready-To-Use IHC Kit. RBX1 primary antibody included.

产品信息

66716-1-PBS targets RBX1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7005

Product name: Recombinant human RBX1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-108 aa of BC001466

Sequence: MAAAMDVDTPSGTNSGAGKKRFEVKKWNAVALWAWDIVVDNCAICRNHIMDLCIECQANQASATSEECTVAWGVCNHAFHFHCISRWLKTRQVCPLDNREWEFQKYGH

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ring-box 1
别名 Protein ZYP, EC:2.3.2.27, E3 ubiquitin-protein transferase RBX1, E3 ubiquitin-protein ligase RBX1, N-terminally processed, E3 ubiquitin-protein ligase RBX1
计算分子量 12 kDa
观测分子量 12-15 kDa
GenBank蛋白编号BC001466
基因名称 RBX1
Gene ID (NCBI) 9978
RRIDAB_2882067
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP62877
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RBX1(RING-box protein 1) is also named as RNF75, ROC1 and is a requisite component of the multi- subunit SCF family E3s36-40. It mediates the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair.

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