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验证数据展示

  • WB analysis using 85086-5-RR (same clone as 85086-5-PBS)

    WB analysis using 85086-5-RR (same clone as 85086-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85086-5-RR (RBPMS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85086-5-PBS in a different storage buffer formulation.

  • IHC staining of mouse eye using 85086-5-RR (same clone as 85086-5-PBS)

    IHC staining of mouse eye using 85086-5-RR (same clone as 85086-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 85086-5-RR (RBPMS antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85086-5-PBS in a different storage buffer formulation.

  • IHC staining of mouse eye using 85086-5-RR (same clone as 85086-5-PBS)

    IHC staining of mouse eye using 85086-5-RR (same clone as 85086-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 85086-5-RR (RBPMS antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85086-5-PBS in a different storage buffer formulation.

  • IF Staining of mouse retina using 85086-5-RR (same clone as 85086-5-PBS)

    IF Staining of mouse retina using 85086-5-RR (same clone as 85086-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse retina tissue using RBPMS antibody (85086-5-RR, Clone: 242730B7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85086-5-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 85086-5-RR (same clone as 85086-5-PBS)

    IF Staining of HepG2 using 85086-5-RR (same clone as 85086-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RBPMS antibody (85086-5-RR, Clone: 242730B7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85086-5-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 85086-5-RR (same clone as 85086-5-PBS)

    FC experiment of A549 using 85086-5-RR (same clone as 85086-5-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug RBPMS Recombinant antibody (85086-5-RR, Clone:242730B7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 85086-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85086-5-RR

    Affinity and Kinetic Characterization of 85086-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85086-5-RR against Human RBPMS were performed. The affinity constant is 0.153 nM.

RBPMS Recombinant monoclonal antibody, PBS Only

RBPMS Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA
Cat No. 85086-5-PBS

产品说明书

CloneNo. 242730B7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

别名

242730B7, Hermes, RBP MS, RBP-MS, RNA-binding protein with multiple splicing

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85086-5-PBS targets RBPMS in WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7256

Product name: Recombinant human RBPMS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-219 aa of BC003608

Sequence: MNNGGKAEKENTPSEANLQEEEVRTLFVSGLPLDIKPRELYLLFRPFKGYEGSLIKLTSKQPVGFVSFDSRSEAEAAKNALNGIRFDPEIPQTLRLEFAKANTKMAKNKLVGTPNPSTPLPNTVPQFIAREPYELTVPALYPSSPEVWAPYPLYPAELAPALPPPAFTYPASLHAQCFSPEAKPNTPVFCPLLQQIRFVSGNVFVTYQPTADQQRELPC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RNA binding protein with multiple splicing
别名 242730B7, Hermes, RBP MS, RBP-MS, RNA-binding protein with multiple splicing
计算分子量 22 kDa
观测分子量28-30 kDa
GenBank蛋白编号BC003608
基因名称 RBPMS
Gene ID (NCBI) 11030
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ93062
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RBPMS is a member of the RNA recognition motif family of RNA-binding proteins.It has a single, putative RNA recognition motif in its N-terminus.

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