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验证数据展示

  • WB analysis of HEK-293 using 14541-1-AP (same clone as 14541-1-PBS)

    WB analysis of HEK-293 using 14541-1-AP (same clone as 14541-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14541-1-AP (RBP7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14541-1-PBS in a different storage buffer formulation.

  • IF Staining of human kidney using 14541-1-AP (same clone as 14541-1-PBS)

    IF Staining of human kidney using 14541-1-AP (same clone as 14541-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using RBP7 antibody (14541-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14541-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 14541-1-AP (same clone as 14541-1-PBS)

    IP experiment of HEK-293 using 14541-1-AP (same clone as 14541-1-PBS)

    IP result of anti-RBP7 (IP:14541-1-AP, 4ug; Detection:14541-1-AP 1:500) with HEK-293 cells lysate 3000ug. This data was developed using the same antibody clone with 14541-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse adipose using 14541-1-AP (same clone as 14541-1-PBS)

    WB analysis of mouse adipose using 14541-1-AP (same clone as 14541-1-PBS)

    mouse adipose tissue were subjected to SDS PAGE followed by western blot with 14541-1-AP (RBP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14541-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 14541-1-AP (same clone as 14541-1-PBS)

    IHC staining of human colon cancer using 14541-1-AP (same clone as 14541-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14541-1-AP (RBP7 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14541-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 14541-1-AP (same clone as 14541-1-PBS)

    IF Staining of HEK-293 using 14541-1-AP (same clone as 14541-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using RBP7 antibody (14541-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14541-1-PBS in a different storage buffer formulation.

RBP7 Polyclonal antibody, PBS Only

RBP7 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA
Cat No. 14541-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

别名

Cellular retinoic acid-binding protein 4, Cellular retinoic acid-binding protein IV, CRABP IV, CRABP4, CRABP-IV

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

14541-1-PBS targets RBP7 in WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag6045

Product name: Recombinant human RBP7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-134 aa of BC063013

Sequence: MPADLSGTWTLLSSDNFEGYMLALGIDFATRKIAKLLKPQKVIEQNGDSFTIHTNSSLRNYFVKFKVGEEFDEDNRGLDNRKCKSLVIWDNDRLTCIQKGEKKNRGWTHWIEGDKLHLEMFCEGQVCKQTFQRA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 retinol binding protein 7, cellular
别名 Cellular retinoic acid-binding protein 4, Cellular retinoic acid-binding protein IV, CRABP IV, CRABP4, CRABP-IV
计算分子量 15.5 kDa
观测分子量 15-18 kDa
GenBank蛋白编号BC063013
基因名称 RBP7
Gene ID (NCBI) 116362
RRIDAB_2179293
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96R05
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RBP7(Retinol Binding Protein 7), also known as CRBP4 or CRBP4, belongs to the cell retinol binding protein (CRBP) family. Its structure is a typical β-barrel, which can bind all-trans retinol, but its affinity is lower than other CRBP members. RBP7 is mainly located in cytoplasm, and has secretory characteristics, and is highly expressed in adipose tissue (white/brown), kidney, heart and other tissues. Functionally, it is responsible for the stability and transport of retinol in cells and provides substrates for LRAT to form retinyl esters. At the same time, it is regulated by PPARγ, which promotes preadipocyte differentiation and triglyceride accumulation, and mediates vascular endothelial protection through adiponectin. In terms of diseases, the expression of RBP7 is up-regulated in many hepatocellular carcinoma cell lines, but the level is low in normal hepatocytes L02. As a key chaperone protein of retinol metabolic pathway, RBP7 is a potential target for metabolic diseases and tumor research.

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