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验证数据展示

  • WB analysis using 67395-1-Ig

    WB analysis using 67395-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67395-1-Ig (RBMS2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 67395-1-Ig

    IHC staining of human placenta using 67395-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67395-1-Ig (RBMS2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse adrenal gland using 67395-1-Ig

    IHC staining of mouse adrenal gland using 67395-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue slide using 67395-1-Ig (RBMS2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 67395-1-Ig

    IHC staining of mouse skeletal muscle using 67395-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67395-1-Ig (RBMS2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RBMS2 Monoclonal antibody

RBMS2 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67395-1-Ig

产品说明书

CloneNo. 1E5C5

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

1E5C5, RNA-binding motif, single-stranded-interacting protein 2, SCR3, Suppressor of CDC2 with RNA-binding motif 3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inNCI-H1299 cells, U2OS cells, LNCaP cells, A549 cells, HeLa cells
Positive IHC detected inhuman placenta tissue, mouse adrenal gland tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67395-1-Ig targets RBMS2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag27721

Product name: Recombinant human RBMS2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-407 aa of BC027863

Sequence: MLLSVTSRPGISTFGYNRNNKKPYVSLAQQMAPPSPSNSTPNSSSGSNGNDQLSKTNLYIRGLQPGTTDQDLVKLCQPYGKIVSTKAILDKTTNKCKGYGFVDFDSPSAAQKAVTALKASGVQAQMAKQQEQDPTNLYISNLPLSMDEQELEGMLKPFGQVISTRILRDTSGTSRGVGFARMESTEKCEAIITHFNGKYIKTPPGVPAPSDPLLCKFADGGPKKRQNQGKFVQNGRAWPRNADMGVMALTYDPTTALQNGFYPAPYNITPNRMLAQSALSPYLSSPVSSYQRVTQTSPLQVPNPSWMHHHSYLMQPSGSVLTPGMDHPISLQPASMMGPLTQQLGHLSLSSTGTYMPTAAAMQGAYISQYTPVPSSSVSVEESSGQQNQVAVDAPSEHGVYSFQFNK

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 RNA binding motif, single stranded interacting protein 2
别名 1E5C5, RNA-binding motif, single-stranded-interacting protein 2, SCR3, Suppressor of CDC2 with RNA-binding motif 3
计算分子量 407 aa, 44 kDa
观测分子量 44 kDa
GenBank蛋白编号BC027863
基因名称 RBMS2
Gene ID (NCBI) 5939
RRIDAB_2882639
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ15434
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RBMS2 (RNA-binding motif, single-stranded-interacting protein 2), also known as SCR3 (Suppressor of CDC2 with RNA-binding motif 3), is a member of c-Myc single-strand binding proteins (MSSPs) family and encodes RNA binding protein. RBMS2 plays a critical role in the regulation of transcription and alternative splicing, both key cellular processes related to cell proliferation and programmed cell death or apoptosis (PMID: 40854446). Overexpression of RBMS2 inhibited CRC cell proliferation, migration, and epithelial-mesenchymal transition (EMT) (PMID: 30514345, 38058408).

实验方案

Product Specific Protocols
IHC protocol for RBMS2 antibody 67395-1-IgDownload protocol
WB protocol for RBMS2 antibody 67395-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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