验证数据展示

WB analysis using 14958-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IHC staining of human brain using 14958-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:100 (under 40x lens).
Ready-to-use IHC kits available with RBM8A antibody included
IP experiment of HeLa using 14958-1-AP
IP result of anti-RBM8A,Y14 (IP:14958-1-AP, 4ug; Detection:14958-1-AP 1:500) with HeLa cells lysate 3200ug.
WB analysis using 14958-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14958-1-AP
WB result of RBM8A,Y14 antibody (14958-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM8A,Y14 transfected HeLa cells.
WB analysis of HeLa using 14958-1-AP
WB result of RBM8A,Y14 antibody (14958-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM8A,Y14 transfected HeLa cells.
IF Staining of HeLa using 14958-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBM8A,Y14 antibody (14958-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HeLa using 14958-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBM8A,Y14 antibody (14958-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
IF Staining of HeLa using 14958-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO验证

RBM8A,Y14 Polyclonal antibody

RBM8A,Y14 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 14958-1-AP

产品说明书

引用文献(5)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

别名

RBM8A, BOV-1, BOV 1C, BOV 1B, BOV 1A

缓冲液配方: PBS and Azide

PBS and Azide

偶联物: Unconjugated

Unconjugated

规格:

-/ -

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概述

经过测试的应用
产品信息
发表文章 (5)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	HEK-293T cells, Jurkat cells, HeLa cells, MDA-MB-453s cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human brain tissue, human spleen tissue, human testis tissue, human ovary tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Planning an IHC experiment? We recommend our IHCeasy RBM8A Ready-To-Use IHC Kit. RBM8A primary antibody included.

应用	推荐稀释比
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 1 publications below
WB	See 4 publications below
IHC	See 1 publications below
IF	See 2 publications below

产品信息

14958-1-AP targets RBM8A,Y14 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用	WB, IHC, IF
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse
免疫原	RBM8A,Y14 fusion protein Ag6800 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	RNA binding motif protein 8A
别名	RBM8A, BOV-1, BOV 1C, BOV 1B, BOV 1A
计算分子量	20 kDa
观测分子量	24 kDa
GenBank蛋白编号	BC017088
基因名称	RBM8A
Gene ID (NCBI)	9939
RRID	AB_2178820
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	Q9Y5S9
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

实验方案

Product Specific Protocols
WB protocol for RBM8A,Y14 antibody 14958-1-AP	Download protocol
IHC protocol for RBM8A,Y14 antibody 14958-1-AP	Download protocol
IF protocol for RBM8A,Y14 antibody 14958-1-AP	Download protocol
IP protocol for RBM8A,Y14 antibody 14958-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	WB	Cell Metab NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis. Authors - Mi Kyung Park View Article
mouse	IF	RNA An atlas of chromatoid body components. Authors - Oliver Meikar View Article
human	WB,IHC	Front Oncol RBM8A Promotes Glioblastoma Growth and Invasion Through the Notch/STAT3 Pathway. Authors - Yan Lin KD Validated View Article
human	WB	Cell Death Discov CircEIF3H-IGF2BP2-HuR scaffold complex promotes TNBC progression via stabilizing HSPD1/RBM8A/G3BP1 mRNA. Authors - Xiaojin Song View Article
human	WB,IF	Cell Rep The RNA-binding protein EIF4A3 promotes axon development by direct control of the cytoskeleton Authors - Fernando C Alsina View Article

验证数据展示

WB analysis using 14958-1-AP

IHC staining of human brain using 14958-1-AP

IP experiment of HeLa using 14958-1-AP

WB analysis using 14958-1-AP

WB analysis of HeLa using 14958-1-AP

WB analysis of HeLa using 14958-1-AP

IF Staining of HeLa using 14958-1-AP

IF Staining of HeLa using 14958-1-AP

IF Staining of HeLa using 14958-1-AP

RBM8A,Y14 Polyclonal antibody

RBM8A,Y14 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

实验方案

发表文章

NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.

An atlas of chromatoid body components.

RBM8A Promotes Glioblastoma Growth and Invasion Through the Notch/STAT3 Pathway.

CircEIF3H-IGF2BP2-HuR scaffold complex promotes TNBC progression via stabilizing HSPD1/RBM8A/G3BP1 mRNA.

The RNA-binding protein EIF4A3 promotes axon development by direct control of the cytoskeleton