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验证数据展示

  • WB analysis of HeLa using 60292-1-Ig (same clone as 60292-1-PBS)

    WB analysis of HeLa using 60292-1-Ig (same clone as 60292-1-PBS)

    WB result of RBM4 antibody (60292-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM4 transfected HeLa cells. This data was developed using the same antibody clone with 60292-1-PBS in a different storage buffer formulation.

  • WB analysis using 60292-1-Ig (same clone as 60292-1-PBS)

    WB analysis using 60292-1-Ig (same clone as 60292-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60292-1-Ig (RBM4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60292-1-PBS in a different storage buffer formulation.

  • IHC staining of human cervical cancer using 60292-1-Ig (same clone as 60292-1-PBS)

    IHC staining of human cervical cancer using 60292-1-Ig (same clone as 60292-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60292-1-Ig (RBM4 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60292-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 60292-1-Ig (same clone as 60292-1-PBS)

    IF Staining of HeLa using 60292-1-Ig (same clone as 60292-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 60292-1-Ig(RBM4 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60292-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

RBM4 Monoclonal antibody, PBS Only

RBM4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 60292-1-PBS

产品说明书

CloneNo. 3H3F12

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

3H3F12, hLark, Lark homolog, RBM4A, RNA binding motif protein 4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

60292-1-PBS targets RBM4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 RBM4 fusion protein Ag2190 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 RNA binding motif protein 4
别名 3H3F12, hLark, Lark homolog, RBM4A, RNA binding motif protein 4
计算分子量 40 kDa
观测分子量 40 kDa
GenBank蛋白编号BC021120
基因名称 RBM4
Gene ID (NCBI) 5936
RRIDAB_2881407
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9BWF3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RBM4, RNA-binding motif protein4, contains 2 RRM-type RNA-binding motif and a retroviral-type(RT) zinc finger. RNA-binding factor participates in number of aspects of cellular processes such as alternative splicing of pre-mRNA and translation regulation. RBM4 recruits eIF4A1 to stimulate IRES-dependent translation in responds to cellular stress. Once assembled at the rHRE, the HIF2A-RBM4-eIF4E2 complex captures the 5-prime cap and targets mRNAs to polysomes for active translation, thereby evading hypoxia-induced repression of protein synthesis.