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验证数据展示

  • WB analysis of HeLa using 60292-1-Ig

    WB analysis of HeLa using 60292-1-Ig

    WB result of RBM4 antibody (60292-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM4 transfected HeLa cells.

  • WB analysis using 60292-1-Ig

    WB analysis using 60292-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60292-1-Ig (RBM4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 60292-1-Ig

    IHC staining of human cervical cancer using 60292-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60292-1-Ig (RBM4 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 60292-1-Ig

    IF Staining of HeLa using 60292-1-Ig

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 60292-1-Ig(RBM4 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Featured Product
  • KD/KO验证

RBM4 Monoclonal antibody

RBM4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 60292-1-Ig

产品说明书

CloneNo. 3H3F12

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

3H3F12, hLark, Lark homolog, RBM4A, RNA binding motif protein 4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMCF-7 cells, fetal human brain tissue, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman cervical cancer tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®555 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60292-1-Ig targets RBM4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 RBM4 fusion protein Ag2190 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 RNA binding motif protein 4
别名 3H3F12, hLark, Lark homolog, RBM4A, RNA binding motif protein 4
计算分子量 40 kDa
观测分子量 40 kDa
GenBank蛋白编号BC021120
基因名称 RBM4
Gene ID (NCBI) 5936
RRIDAB_2881407
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9BWF3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RBM4, RNA-binding motif protein4, contains 2 RRM-type RNA-binding motif and a retroviral-type(RT) zinc finger. RNA-binding factor participates in number of aspects of cellular processes such as alternative splicing of pre-mRNA and translation regulation. RBM4 recruits eIF4A1 to stimulate IRES-dependent translation in responds to cellular stress. Once assembled at the rHRE, the HIF2A-RBM4-eIF4E2 complex captures the 5-prime cap and targets mRNAs to polysomes for active translation, thereby evading hypoxia-induced repression of protein synthesis.

实验方案

Product Specific Protocols
WB protocol for RBM4 antibody 60292-1-IgDownload protocol
IHC protocol for RBM4 antibody 60292-1-IgDownload protocol
IF protocol for RBM4 antibody 60292-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols