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验证数据展示

  • WB analysis using RMX00066

    WB analysis using RMX00066

    Various lysates were subjected to SDS PAGE followed by western blot with RMX00066 (RBM25 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using RMX00066

    WB analysis using RMX00066

    Various lysates were subjected to SDS PAGE followed by western blot with RMX00066 (RBM25 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using RMX00066

    IP experiment of HEK-293 using RMX00066

    IP result of anti-RBM25 (IP:RMX00066, 4ug; Detection:RMX00066 1:5000) with HEK-293 cells lysate 1000 ug.

  • IHC staining of human colon cancer using RMX00066

    IHC staining of human colon cancer using RMX00066

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using RMX00066 (RBM25 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using RMX00066

    IHC staining of human colon cancer using RMX00066

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using RMX00066 (RBM25 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using RMX00066

    IF Staining of HeLa using RMX00066

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBM25 antibody (RMX00066) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Multi-rAb® RBM25 Multi-Recombinant antibody

RBM25 Multi-Recombinant Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. RMX00066

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

Arg/Glu/Asp-rich protein of 120 kDa, NET52, Protein S164, RBM 25, RED120

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, PC-12 cells, Jurkat cells, mouse brain tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

RMX00066 targets RBM25 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

Recombinant protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Multi-Recombinant
产品类型 Antibody
全称 RNA binding motif protein 25
别名 Arg/Glu/Asp-rich protein of 120 kDa, NET52, Protein S164, RBM 25, RED120
计算分子量 843 aa, 100 kDa
观测分子量100-120 kDa
GenBank蛋白编号BC113389
基因名称 RBM25
Gene ID (NCBI) 58517
偶联类型 Unconjugated
形式Liquid
纯化方式N/A
UNIPROT IDP49756
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNA-binding protein 25 (RBM25), also named as Protein S164 or RNPC7, is a 843 amino acid protein, which contains one RRM domain and one PWI domain. RBM25 localizes in the nucleus and cytoplasm. RBM25 acts as a regulator of alternative pre-mRNA splicing and is involved in apoptotic cell death through the regulation of the apoptotic factor BCL2L1 isoform expression.

实验方案

Product Specific Protocols
IF protocol for RBM25 antibody RMX00066Download protocol
IHC protocol for RBM25 antibody RMX00066Download protocol
IP protocol for RBM25 antibody RMX00066Download protocol
WB protocol for RBM25 antibody RMX00066Download protocol
Standard Protocols
Click here to view our Standard Protocols
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