RBM25 Polyclonal antibody

RBM25 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 25297-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

Arg/Glu/Asp-rich protein of 120 kDa, NET52, Protein S164, RBM 25, RED120

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, mouse brain tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25297-1-AP targets RBM25 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IP, RIP
经测试反应性 human, mouse
文献引用反应性human, mouse, rat
免疫原 RBM25 fusion protein Ag18244 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RNA binding motif protein 25
别名 Arg/Glu/Asp-rich protein of 120 kDa, NET52, Protein S164, RBM 25, RED120
计算分子量 843 aa, 100 kDa
观测分子量 110-120 kDa
GenBank蛋白编号BC113389
基因名称 RBM25
Gene ID (NCBI) 58517
RRIDAB_2880014
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP49756
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNA-binding protein 25 (RBM25), also named as Protein S164 or RNPC7, is a 843 amino acid protein, which contains one RRM domain and one PWI domain. RBM25 localizes in the nucleus and cytoplasm. RBM25 acts as a regulator of alternative pre-mRNA splicing and is involved in apoptotic cell death through the regulation of the apoptotic factor BCL2L1 isoform expression.

实验方案

Product Specific Protocols
WB protocol for RBM25 antibody 25297-1-APDownload protocol
IHC protocol for RBM25 antibody 25297-1-APDownload protocol
IF protocol for RBM25 antibody 25297-1-APDownload protocol
IP protocol for RBM25 antibody 25297-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP

Cell Insight

LUC7L3 is a downstream factor of SRSF1 and prevents genomic instability

Authors - Xiaqing Zhang
mouseWB

Gene

ZC3H13 may participate in the ferroptosis process of sepsis-induced cardiomyopathy by regulating the expression of Pnn and Rbm25

Authors - Wenji Lin
humanWB,IHC

Sci China Life Sci

RBM25 depletion suppresses the growth of colon cancer cells through regulating alternative splicing of MNK2

Authors - Lili Zhi
  • KD Validated
mouseRIP

Arterioscler Thromb Vasc Biol

Super Enhancer-Associated Circular RNA-CircKrt4 Regulates Hypoxic Pulmonary Artery Endothelial Cell Dysfunction in Mice

Authors - Cui Ma
ratIP

PeerJ

RBM25 binds to and regulates alternative splicing levels of Slc38a9, Csf1, and Coro6 to affect immune and inflammatory processes in H9c2 cells

Authors - Xin Tian
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