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验证数据展示

  • WB analysis using 22103-1-AP (same clone as 22103-1-PBS)

    WB analysis using 22103-1-AP (same clone as 22103-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22103-1-AP (RBM22 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22103-1-PBS in a different storage buffer formulation.

  • IHC staining of rat stomach using 22103-1-AP (same clone as 22103-1-PBS)

    IHC staining of rat stomach using 22103-1-AP (same clone as 22103-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 22103-1-AP (RBM22 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22103-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RBM22 antibody included
  • IF Staining of U2OS using 22103-1-AP (same clone as 22103-1-PBS)

    IF Staining of U2OS using 22103-1-AP (same clone as 22103-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RBM22 antibody (22103-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 22103-1-PBS in a different storage buffer formulation.

RBM22 Polyclonal antibody, PBS Only

RBM22 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 22103-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

199G4, fSAP47, Pre mRNA splicing factor RBM22, Pre-mRNA-splicing factor RBM22, RNA binding motif protein 22

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® RBM22 Ready-To-Use IHC Kit. RBM22 primary antibody included.

产品信息

22103-1-PBS targets RBM22 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag17315

Product name: Recombinant human RBM22 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-420 aa of BC003402

Sequence: MATSLGSNTYNRQNWEDADFPILCQTCLGENPYIRMTKEKYGKECKICARPFTVFRWCPGVRMRFKKTEVCQTCSKLKNVCQTCLLDLEYGLPIQVRDAGLSFKDDMPKSDVNKEYYTQNMEREISNSDGTRPVGMLGKATSTSDMLLKLARTTPYYKRNRPHICSFWVKGECKRGEECPYRHEKPTDPDDPLADQNIKDRYYGINDPVADKLLKRASTMPRLDPPEDKTITTLYVGGLGDTITETDLRNHFYQFGEIRTITVVQRQQCAFIQFATRQAAEVAAEKSFNKLIVNGRRLNVKWGRSQAARGKEKEKDGTTDSGIKLEPVPGLPGALPPPPAAEEEASANYFNLPPSGPPAVVNIALPPPPGIAPPPPPGFGPHMFHPMGPPPPFMRAPGPIHYPSQDPQRMGAHAGKHSSP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RNA binding motif protein 22
别名 199G4, fSAP47, Pre mRNA splicing factor RBM22, Pre-mRNA-splicing factor RBM22, RNA binding motif protein 22
计算分子量 420 aa, 47 kDa
观测分子量 47-50 kDa
GenBank蛋白编号BC003402
基因名称 RBM22
Gene ID (NCBI) 55696
RRIDAB_2878996
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NW64
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RBM22 is mainly involved in pre-mRNA splicing, playing the essential role of maintaining the conformation of the catalytic core of the spliceosome and acting as a bridge between the catalytic core and other essential protein components of the spliceosome. RBM22 is also involved in gene regulation, and is able to bind DNA, acting as a bona fide transcription factor on a large number of target genes. Due to its wide scope in the regulation of gene expression, RBM22 has been associated with several pathologies and, notably, with the aggressiveness of cancer cells and with the phenotype of a myelodysplastic syndrome. (PMID: 35158909)

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