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验证数据展示

  • WB analysis using 82889-3-RR (same clone as 82889-3-PBS)

    WB analysis using 82889-3-RR (same clone as 82889-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82889-3-RR (RBM17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82889-3-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 82889-3-RR (same clone as 82889-3-PBS)

    WB analysis of MCF-7 using 82889-3-RR (same clone as 82889-3-PBS)

    WB result of RBM17 antibody (82889-3-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM17 transfected MCF-7 cells. This data was developed using the same antibody clone with 82889-3-PBS in a different storage buffer formulation.

  • IP experiment of MCF-7 using 82889-3-RR (same clone as 82889-3-PBS)

    IP experiment of MCF-7 using 82889-3-RR (same clone as 82889-3-PBS)

    IP result of anti-RBM17 (IP:82889-3-RR, 4ug; Detection:82889-3-RR 1:10000) with MCF-7 cells lysate 1470 ug. This data was developed using the same antibody clone with 82889-3-PBS in a different storage buffer formulation.

  • IHC staining of human ovarian cancer using 82889-3-RR (same clone as 82889-3-PBS)

    IHC staining of human ovarian cancer using 82889-3-RR (same clone as 82889-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82889-3-RR (RBM17 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82889-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82889-3-RR

    Affinity and Kinetic Characterization of 82889-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82889-3-RR against Human RBM17 were performed. The affinity constant is 0.828 nM.

  • Featured Product
  • KD/KO验证
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RBM17 Recombinant monoclonal antibody, PBS Only

RBM17 Uni-rAb® Recombinant Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 82889-3-PBS

产品说明书

CloneNo. 230125A3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IP, Indirect ELISA

别名

SPF45, SPF 45, RNA-binding motif protein 17, RBM 17, 45 kDa-splicing factor

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

82889-3-PBS targets RBM17 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag0985

Product name: Recombinant human RBM17 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-308 aa of BC007871

Sequence: MSLYDDLGVETSDSKTEGWSKNFKLLQSQLQVKKAALTQAKSQRTKQSTVLAPVIDLKRGGSSDDRQIVDTPPHVAAGLKDPVPSGFSAGEVLIPLADEYDPMFPNDYEKVVKRQREERQRQRELERQKEIEEREKRRKDRHEASGFARRPDPDSDEDEDYERERRKRSMGGAAIAPPTSLVEKDKELPRDFPYEEDSRPRSQSSKAAIPPPVYEEQDRPRSPTGPSNSFLANMGGTVAHKIMQKYGFREGQGLGKHEQGLSTALSVEKTSKRGGKIIVGDATEKDASKKSDSNPLTEILKCPTKVVL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 RNA binding motif protein 17
别名 SPF45, SPF 45, RNA-binding motif protein 17, RBM 17, 45 kDa-splicing factor
计算分子量 45 kDa
观测分子量45-50 kDa
GenBank蛋白编号BC007871
基因名称 RBM17
Gene ID (NCBI) 84991
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ96I25
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RNA-binding motif protein 17 (RBM17), also named splicing factor 45, was first identified as a member of the spliceosome. RBM17 expression is low in normal tissues but is overexpressed in several forms of cancer, including breast, ovarian and prostate.