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验证数据展示

  • WB analysis of HT-1080 using 18893-1-AP (same clone as 18893-1-PBS)

    WB analysis of HT-1080 using 18893-1-AP (same clone as 18893-1-PBS)

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 18893-1-AP (RBM16 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18893-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 18893-1-AP (same clone as 18893-1-PBS)

    IHC staining of human testis using 18893-1-AP (same clone as 18893-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 18893-1-AP (RBM16 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 18893-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SCAF8 antibody included
  • IP experiment of HeLa using 18893-1-AP (same clone as 18893-1-PBS)

    IP experiment of HeLa using 18893-1-AP (same clone as 18893-1-PBS)

    IP result of anti-RBM16 (IP:18893-1-AP, 4ug; Detection:18893-1-AP 1:800) with HeLa cells lysate 3600ug. This data was developed using the same antibody clone with 18893-1-PBS in a different storage buffer formulation.

  • WB analysis using 18893-1-AP (same clone as 18893-1-PBS)

    WB analysis using 18893-1-AP (same clone as 18893-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18893-1-AP (RBM16 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18893-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 18893-1-AP (same clone as 18893-1-PBS)

    IF Staining of HeLa using 18893-1-AP (same clone as 18893-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBM16 antibody (18893-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 18893-1-PBS in a different storage buffer formulation.

RBM16 Polyclonal antibody, PBS Only

RBM16 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 18893-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy SCAF8 Ready-To-Use IHC Kit. SCAF8 primary antibody included.

产品信息

18893-1-PBS targets RBM16 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag13499

Product name: Recombinant human RBM16 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 921-1271 aa of BC070071

Sequence: MPMLDIRPGLIPQAPGPRFPLIQPGIPPQRGIPPPSVLDSALHPPPRGPFPPGDIFSQPERPFLAPGRQSVDNVTNPEKRIPLGNDNIQQEGDRDYRFPPIETRESISRPPPVDVRDVVGRPIDPREGPGRPPLDGRDHFGRPPVDIRENLVRPGIDHLGRRDHFGFNPEKPWGHRGDFDEREHRVLPVYGGPKGLHEERGRFRSGNYRFDPRSGPWNRGFGQEVHRDFDDRRRPWERQRDRDDRDFDFCREMNGNRLGRDRIQNTWVPPPHARVFDYFEGATSQRKGDNVPQVNGENTERHAQPPPIPVQNDPELYEKLTSSNEINKEKSDTVADIESEPVVESTETEGT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RNA binding motif protein 16
别名
计算分子量 1271 aa, 141 kDa
观测分子量 170 kDa
GenBank蛋白编号BC070071
基因名称 RBM16
Gene ID (NCBI) 22828
RRIDAB_2175908
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UPN6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RBM16, also named as SCAF8, CCAP7, KIAA1116, is a 1271 amino acid protein, which interacts with POLR2A. RBM16 may play a role in mRNA processing. Immunoblot analysis of the total rat liver nuclear and nuclear matrix protein fractions revealed a single band with an apparent molecular mass of 170 kDa. While the apparent molecular mass is slightly larger than the 140 kDa predicted from the cDNA sequence, we note that the protein is rich in proline residues (10%) and therefore may display aberrant mobility in SDS gel electrophoresis. (PMID: 9528809 )

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