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验证数据展示

  • WB analysis using 67506-1-Ig (same clone as 67506-1-PBS)

    WB analysis using 67506-1-Ig (same clone as 67506-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67506-1-Ig (RBM15B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67506-1-PBS in a different storage buffer formulation.

  • WB analysis using 67506-1-Ig (same clone as 67506-1-PBS)

    WB analysis using 67506-1-Ig (same clone as 67506-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 67506-1-Ig (RBM15B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67506-1-PBS in a different storage buffer formulation.

  • WB analysis using 67506-1-Ig (same clone as 67506-1-PBS)

    WB analysis using 67506-1-Ig (same clone as 67506-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67506-1-Ig (RBM15B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67506-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 67506-1-Ig (same clone as 67506-1-PBS)

    IHC staining of human colon cancer using 67506-1-Ig (same clone as 67506-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67506-1-Ig (RBM15B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67506-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RBM15B antibody included
  • IHC staining of human colon using 67506-1-Ig (same clone as 67506-1-PBS)

    IHC staining of human colon using 67506-1-Ig (same clone as 67506-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67506-1-Ig (RBM15B antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67506-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RBM15B antibody included
  • IHC staining of rat colon using 67506-1-Ig (same clone as 67506-1-PBS)

    IHC staining of rat colon using 67506-1-Ig (same clone as 67506-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67506-1-Ig (RBM15B antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67506-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RBM15B antibody included
  • IHC staining of rat colon using 67506-1-Ig (same clone as 67506-1-PBS)

    IHC staining of rat colon using 67506-1-Ig (same clone as 67506-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 67506-1-Ig (RBM15B antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67506-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RBM15B antibody included

RBM15B Monoclonal antibody, PBS Only

RBM15B Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67506-1-PBS

产品说明书

CloneNo. 1C2C11

宿主/亚型

Mouse / IgG1

种属反应性

human, rat

应用

WB, IHC, Indirect ELISA

别名

1C2C11, HsOTT3, HUMAGCGB, HuOTT3, One-twenty two protein 3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy RBM15B Ready-To-Use IHC Kit. RBM15B primary antibody included.

产品信息

67506-1-PBS targets RBM15B in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag17853

Product name: Recombinant human RBM15B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 490-769 aa of BC001367

Sequence: AKAEETRYPQQYQPSPLPVHYELLTDGYTRHRNLDADLVRDRTPPHLLYSDRDRTFLEGDWTSPSKSSDRRNSLEGYSRSVRSRSGERWGADGDRGLPKPWEERRKRRSLSSDRGRTTHSPYEERSRTKGSGQQSERGSDRTPERSRKENHSSEGTKESSSNSLSNSRHGAEERGHHHHHHEAADSSHGKKARDSERNHRTTEAEPKPLEEPKHETKKLKNLSEYAQTLQLGWNGLLVLKNSCFPTSMHILEGDQGVISSLLKDHTSGSKLTQLKIAQRL

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 RNA binding motif protein 15B
别名 1C2C11, HsOTT3, HUMAGCGB, HuOTT3, One-twenty two protein 3
计算分子量 890 aa, 97 kDa
观测分子量 105 kDa
GenBank蛋白编号BC001367
基因名称 RBM15B
Gene ID (NCBI) 29890
RRIDAB_2882729
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8NDT2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RBM15B, one members of the SPEN (Split-end) family of proteins, have repressor function in several signaling pathways and may bind to RNA through interaction with spliceosome components. Present polyclonal anti-RBM15B antibody was produced by immunizing animals with C-terminus of RBM15B, which homolog with a short form of RBM15B(563aa, ~70kDa).