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CoraLite®594-conjugated RBM15 Monoclonal antibody
RBM15 Monoclonal Antibody for FC (Intra), IF/ICC
Host / Isotype
Mouse / IgG2a
Reactivity
human
Applications
FC (Intra), IF/ICC
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
4A1A4
验证数据展示
经过测试的应用
Positive IF detected in | HeLa cells |
Positive FC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy RBM15 Ready-To-Use IHC Kit. RBM15 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66059-1-Ig
推荐稀释比
Application | Dilution |
---|---|
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
The immunogen of CL594-66059 is RBM15 Fusion Protein expressed in E. coli.
Tested Applications | FC (Intra), IF/ICC |
Tested Reactivity | human |
Immunogen | RBM15 fusion protein Ag17970 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | RNA binding motif protein 15 |
Synonyms | FLJ12479, FLJ21943, One twenty two protein 1, OTT, OTT1, RBM15, RNA binding motif protein 15, SPEN |
Calculated Molecular Weight | 105 kDa |
Observed Molecular Weight | 107 kDa |
GenBank Accession Number | BC006397 |
Gene Symbol | RBM15 |
Gene ID (NCBI) | 64783 |
RRID | AB_2919943 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q96T37 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RNA-binding motif protein 15 (RBM15) belongs to the SPEN protein family, which have repressor function in several signaling pathway, and may bind to RNA through interaction with spliceosome components. It was first described as a 5′ translocation partner of the MAL gene in t(1,22)(p13;q13) infant acute megakaryocytic leukemia, and it functions in murine hematopoiesis through modulating Notch-induced transcriptional activation, which play crucial roles in leukemogenesis. Decreasing RBM15 levels with RNA interference could inhibit the growth and proliferation, block the cell cycle, and induce apoptosis in CML cells.
实验方案
Product Specific Protocols | |
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IF protocol for CL594 RBM15 antibody CL594-66059 | Download protocol |
FC protocol for CL594 RBM15 antibody CL594-66059 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |