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验证数据展示

  • IF Staining of HepG2 using CL594-10587

    IF Staining of HepG2 using CL594-10587

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 RBM15 antibody (CL594-10587) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated RBM15 Polyclonal antibody

RBM15 Polyclonal Antibody for IF/ICC
Cat No. CL594-10587

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC

别名

FLJ12479, FLJ21943, One twenty two protein 1, OTT, OTT1, RBM15, RNA binding motif protein 15, SPEN

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy RBM15 Ready-To-Use IHC Kit. RBM15 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10587-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-10587 targets RBM15 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 RBM15 fusion protein Ag0938 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RNA binding motif protein 15
别名 FLJ12479, FLJ21943, One twenty two protein 1, OTT, OTT1, RBM15, RNA binding motif protein 15, SPEN
计算分子量 105 kDa
观测分子量 100-107 kDa
GenBank蛋白编号BC006397
基因名称 RBM15
Gene ID (NCBI) 64783
RRIDAB_2919738
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96T37
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNA-binding motif protein 15 (RBM15) belongs to the SPEN protein family, which have repressor function in several signaling pathway, and may bind to RNA through interaction with spliceosome components. It was first described as a 5′ translocation partner of the MAL gene in t(1,22)(p13;q13) infant acute megakaryocytic leukemia, and it functions in murine hematopoiesis through modulating Notch-induced transcriptional activation, which play crucial roles in leukemogenesis. Decreasing RBM15 levels with RNA interference could inhibit the growth and proliferation, block the cell cycle, and induce apoptosis in CML cells.

实验方案

Product Specific Protocols
IF protocol for CL594 RBM15 antibody CL594-10587Download protocol
Standard Protocols
Click here to view our Standard Protocols