验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IF-P detected in | mouse brain tissue |
Planning an IHC experiment? We recommend our IHCeasy RBM10 Ready-To-Use IHC Kit. RBM10 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14423-1-AP targets RBM10 in WB, IHC, IF-P, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF-P, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, RIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | RBM10 fusion protein Ag5788 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RNA binding motif protein 10 |
| 别名 | RNA binding protein 10, KIAA0122, GPATCH9, GPATC9, G patch domain-containing protein 9 |
| 计算分子量 | 104 kDa |
| 观测分子量 | 115 kDa |
| GenBank蛋白编号 | BC024153 |
| 基因名称 | RBM10 |
| Gene ID (NCBI) | 8241 |
| RRID | AB_2175593 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P98175 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RBM10, also named as RNA-binding protein 10, is a 930 amino acid protein, which is in the extranucleolar nucleoplasm constitutes hundreds of nuclear domains, which dynamically change their structures in a reversible manner. RBM10 may be involved in post-transcriptional processing, most probably in mRNA splicing. RBM10 has some isoforms with MV 100-130 kDa (PMID: 25889998 and PMID: 18315527) .
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RBM10 antibody 14423-1-AP | Download protocol |
| IF protocol for RBM10 antibody 14423-1-AP | Download protocol |
| IP protocol for RBM10 antibody 14423-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A RNA-binding motif protein 10 inactivates c-Myc by partnering with ribosomal proteins uL18 and uL5
| ||
Oncogene RNA-binding motif protein 10 induces apoptosis and suppresses proliferation by activating p53.
| ||
Exp Cell Res RBM10 regulates the tumorigenic potential of human cancer cells by modulating PPM1B and YBX1 activities
| ||
BMC Mol Biol Splicing arrays reveal novel RBM10 targets, including SMN2 pre-mRNA.
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J Cell Mol Med RBM10 inhibits cell proliferation of lung adenocarcinoma via RAP1/AKT/CREB signalling pathway. | ||
Oxid Med Cell Longev RBM10 Is a Biomarker Associated with Pan-Cancer Prognosis and Immune Infiltration: System Analysis Combined with In Vitro and Vivo Experiments
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