验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, Raji cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
产品信息
27251-1-AP targets RBL2 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | RBL2 fusion protein Ag25087 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | retinoblastoma-like 2 (p130) |
| 别名 | P130, pRb2, Rb2, RBL2, RBR 2, retinoblastoma like 2 (p130), Retinoblastoma like protein 2 |
| 计算分子量 | 130 kDa |
| 观测分子量 | 128 kDa |
| GenBank蛋白编号 | BC034490 |
| 基因名称 | RBL2 |
| Gene ID (NCBI) | 5934 |
| RRID | AB_2880819 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q08999 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RBL2, also named as Retinoblastoma-like protein 2 or p130, is a 1130 amino acid protein, which Belongs to the retinoblastoma protein (RB) family. RBL2 is a Key regulator of entry into cell division and is directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. RBL2 recruits and targets histone methyltransferases KMT5B and KMT5C, leading to epigenetic transcriptional repression and controls histone H4 'Lys-20' trimethylation. RBL2 probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. RBL2 may act as a tumor suppressor.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RBL2 antibody 27251-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biochem Pharmacol Ribociclib mitigates cisplatin-associated kidney injury through retinoblastoma-1 dependent mechanisms. | ||
Mol Pharm Synergetic and Antagonistic Molecular Effects Mediated by the Feedback Loop of p53 and JNK between Saikosaponin D and SP600125 on Lung Cancer A549 Cells. | ||
Life Sci Early-senescent bone marrow mesenchymal stem cells promote C2C12 cell myogenic differentiation by preventing the nuclear translocation of FOXO3. | ||
Nat Commun An actin filament branching surveillance system regulates cell cycle progression, cytokinesis and primary ciliogenesis
| ||
