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CoraLite®594-conjugated RBBP9 Monoclonal antibody

RBBP9 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3D5E11

Cat No : CL594-66015

Print datasheet

Synonyms

B5T overexpressed gene protein, BOG, Protein BOG, Putative hydrolase RBBP9, RBBP 10, RBBP 9, RBBP10, RBBP9

验证数据展示

  • FC experiment of HeLa using CL594-66015

    FC experiment of HeLa using CL594-66015

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human RBBP9 (CL594-66015, Clone:3D5E11) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL594-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC detected inHeLa cells

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66015 targets RBBP9 in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra)
Tested Reactivity human
Immunogen RBBP9 fusion protein Ag17864 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name retinoblastoma binding protein 9
Synonyms B5T overexpressed gene protein, BOG, Protein BOG, Putative hydrolase RBBP9, RBBP 10, RBBP 9, RBBP10, RBBP9
Calculated Molecular Weight 186 aa, 21 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC015938
Gene Symbol RBBP9
Gene ID (NCBI) 10741
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75884
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RBBP9, also named as BOG, RBBP10, RBBP-9, RBBP-10 and Protein BOG, belongs to the RBBP9 family. It may play a role in the transformation process due to its capacity to confer resistance to the growth-inhibitory effects of TGF-β1 through interaction with retinoblastoma and the subsequent displacement of E2F-1. RBBP9 is a tumor-associated serine hydrolase activity required for pancreatic neoplasia. It mediates suppression of TGF-β signaling is required for E-cadherin expression as loss of the serine hydrolase activity leads to a reduction in E-cadherin levels and a concomitant decrease in the integrity of tumor cell-cell junctions..RBBP9 protein levels were equivalent in paired primary tumor and nonneoplastic specimens(PMID:20080647)

实验方案

Product Specific Protocols
FC protocol for CL594 RBBP9 antibody CL594-66015Download protocol
Standard Protocols
Click here to view our Standard Protocols