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RBBP9 Monoclonal antibody

RBBP9 Monoclonal Antibody for FC, IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

FC, IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

3D5E11

Cat No : 66015-2-Ig

Print datasheet

Synonyms

BOG, RBBP9

验证数据展示

  • WB analysis using 66015-2-Ig

    WB analysis using 66015-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66015-2-Ig (RBBP9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human pancreas cancer using 66015-2-Ig

    IHC staining of human pancreas cancer using 66015-2-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66015-2-Ig (RBBP9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 66015-2-Ig

    IHC staining of human pancreas cancer using 66015-2-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66015-2-Ig (RBBP9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using 66015-2-Ig

    IF Staining of A549 using 66015-2-Ig

    Immunofluorescent analysis of (10% Formaldehyde ) fixed A549 cells using 66015-2-Ig(RBBP9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • WB analysis using 66015-2-Ig

    WB analysis using 66015-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66015-2-Ig (RBBP9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 66015-2-Ig

    WB analysis of A549 using 66015-2-Ig

    WB result of RBBP9 antibody (66015-2-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBBP9 transfected A549 cells.

  • FC experiment of HeLa using 66015-2-Ig

    FC experiment of HeLa using 66015-2-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human RBBP9 (66015-2-Ig, Clone:3D5E11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive WB detected inpig brain tissue, HeLa cells, A375 cells, A549 cells, rat brain tissue, mouse brain tissue, MCF-7 cells, HepG2 cells, BxPC-3 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells
Positive FC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66015-2-Ig targets RBBP9 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications FC, IF/ICC, IHC, WB, ELISA
Tested Reactivity human, mouse, rat, pig
Immunogen RBBP9 fusion protein Ag17864 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name retinoblastoma binding protein 9
Synonyms BOG, RBBP9
Calculated Molecular Weight 186 aa, 21 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC015938
Gene Symbol RBBP9
Gene ID (NCBI) 10741
RRIDAB_11044197
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75884
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RBBP9, also named as BOG, RBBP10, RBBP-9, RBBP-10 and Protein BOG, belongs to the RBBP9 family. It may play a role in the transformation process due to its capacity to confer resistance to the growth-inhibitory effects of TGF-β1 through interaction with retinoblastoma and the subsequent displacement of E2F-1. RBBP9 is a tumor-associated serine hydrolase activity required for pancreatic neoplasia. It mediates suppression of TGF-β signaling is required for E-cadherin expression as loss of the serine hydrolase activity leads to a reduction in E-cadherin levels and a concomitant decrease in the integrity of tumor cell-cell junctions..RBBP9 protein levels were equivalent in paired primary tumor and nonneoplastic specimens(PMID:20080647)

实验方案

Product Specific Protocols
WB protocol for RBBP9 antibody 66015-2-IgDownload protocol
IHC protocol for RBBP9 antibody 66015-2-IgDownload protocol
IF protocol for RBBP9 antibody 66015-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols