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验证数据展示

  • WB analysis using 66015-2-Ig (same clone as 66015-2-PBS)

    WB analysis using 66015-2-Ig (same clone as 66015-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66015-2-Ig (RBBP9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66015-2-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 66015-2-Ig (same clone as 66015-2-PBS)

    IHC staining of human pancreas cancer using 66015-2-Ig (same clone as 66015-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66015-2-Ig (RBBP9 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66015-2-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 66015-2-Ig (same clone as 66015-2-PBS)

    IHC staining of human pancreas cancer using 66015-2-Ig (same clone as 66015-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66015-2-Ig (RBBP9 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66015-2-PBS in a different storage buffer formulation.

  • WB analysis using 66015-2-Ig (same clone as 66015-2-PBS)

    WB analysis using 66015-2-Ig (same clone as 66015-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66015-2-Ig (RBBP9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66015-2-PBS in a different storage buffer formulation.

  • WB analysis of A549 using 66015-2-Ig (same clone as 66015-2-PBS)

    WB analysis of A549 using 66015-2-Ig (same clone as 66015-2-PBS)

    WB result of RBBP9 antibody (66015-2-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBBP9 transfected A549 cells. This data was developed using the same antibody clone with 66015-2-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 66015-2-Ig (same clone as 66015-2-PBS)

    FC experiment of HeLa using 66015-2-Ig (same clone as 66015-2-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human RBBP9 (66015-2-Ig, Clone:3D5E11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66015-2-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

RBBP9 Monoclonal antibody, PBS Only

RBBP9 Monoclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 66015-2-PBS

产品说明书

CloneNo. 3D5E11

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, pig

应用

WB, IHC, FC (Intra), Indirect ELISA

别名

3D5E11, B5T-overexpressed gene protein, BOG, EC:3.-.-.-, Glutathione S-Transferase

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66015-2-PBS targets RBBP9 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag17864

Product name: Recombinant human RBBP9 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-186 aa of BC015938

Sequence: MASPSKAVIVPGNGGGDVTTHGWYGWVKKELEKIPGFQCLAKNMPDPITARESIWLPFMETELHCDEKTIIIGHSSGAIAAMRYAETHRVYAIVLVSAYTSDLGDENERASGYFTRPWQWEKIKANCPYIVQFGSTDDPFLPWKEQQEVADRLETKLHKFTDCGHFQNTEFHELITVVKSLLKVPA

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 retinoblastoma binding protein 9
别名 3D5E11, B5T-overexpressed gene protein, BOG, EC:3.-.-.-, Glutathione S-Transferase
计算分子量 186 aa, 21 kDa
观测分子量 22 kDa
GenBank蛋白编号BC015938
基因名称 RBBP9
Gene ID (NCBI) 10741
RRIDAB_11044197
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO75884
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RBBP9, also named as BOG, RBBP10, RBBP-9, RBBP-10 and Protein BOG, belongs to the RBBP9 family. It may play a role in the transformation process due to its capacity to confer resistance to the growth-inhibitory effects of TGF-β1 through interaction with retinoblastoma and the subsequent displacement of E2F-1. RBBP9 is a tumor-associated serine hydrolase activity required for pancreatic neoplasia. It mediates suppression of TGF-β signaling is required for E-cadherin expression as loss of the serine hydrolase activity leads to a reduction in E-cadherin levels and a concomitant decrease in the integrity of tumor cell-cell junctions..RBBP9 protein levels were equivalent in paired primary tumor and nonneoplastic specimens(PMID:20080647)

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