CoraLite®594-conjugated RASGRP3 Monoclonal antibody
RASGRP3 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG2a
Reactivity
Human, mouse
Applications
IF/ICC
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
1A10G4
验证数据展示
经过测试的应用
| Positive IF detected in | HEK-293 cells |
For other applications, we recommend the unconjugated version of this antibody, 66650-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-66650 targets RASGRP3 in IF applications and shows reactivity with Human, mouse samples.
| Tested Applications | IF/ICC |
| Tested Reactivity | Human, mouse |
| Immunogen | RASGRP3 fusion protein Ag3810 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | RAS guanyl releasing protein 3 (calcium and DAG-regulated) |
| Synonyms | GRP3, KIAA0846, Ras guanyl releasing protein 3, RASGRP3 |
| Calculated Molecular Weight | 690 aa, 78 kDa |
| Observed Molecular Weight | 78 kDa |
| GenBank Accession Number | BC027849 |
| Gene Symbol | RASGRP3 |
| Gene ID (NCBI) | 25780 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8IV61 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AS guanyl releasing protein 3 (RasGRP3) is a member of the RasGRP family and is able to promote guanine nucleotide exchange of Ha-Ras, R-Ras and Rap1 (PMID: 9582122). RasGRP3 is involved in a diverse range of important biological processes. RasGRP3 has emerged as an important mediator of signaling downstream from receptor coupled phosphoinositide turnover in B and T cells. Overexpression of RasGRP3 is commonly associated with many malignancies, such as pre-Bcell leukemia, Burkitt's lymphoma and natural killer-like T-cell leukemia. Recently, it has been reported that upregulation of RASGRP3 expression in prostate cancer correlates with aggressive capabilities and predicts biochemical recurrence after radical prostatectomy (PMID: 24418912).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 RASGRP3 antibody CL594-66650 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |