RASGRP2 Polyclonal antibody

RASGRP2 Polyclonal Antibody for WB, FC (Intra), ELISA
Cat No. 30189-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA and More (1)

GRP2, F25B3.3 kinase-like protein, Cdc25-like protein, CDC25L, CalDAG-GEFI

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inJurkat cells, MOLT-4 cells, Raji cells
Positive FC (Intra) detected inJurkat cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30189-1-AP targets RASGRP2 in WB, FC (Intra), CoIP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
文献引用应用WB, CoIP
经测试反应性 human
文献引用反应性human
免疫原 RASGRP2 fusion protein Ag32762 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAS guanyl releasing protein 2 (calcium and DAG-regulated)
别名 GRP2, F25B3.3 kinase-like protein, Cdc25-like protein, CDC25L, CalDAG-GEFI
计算分子量69kd
观测分子量69 kDa
GenBank蛋白编号NM_153819
基因名称 RASGRP2
Gene ID (NCBI) 10235
RRIDAB_3086257
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7LDG7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RASGRP2, also named as CDC25L and MCG7, belongs to the RASGRP family. It functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. RASGRP2 may also activate other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. RASGRO2 functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. It may function in the muscarinic acetylcholine receptor M1/CHRM1 signaling pathway. The antibody recognizes the C-term of RASGRP2.

实验方案

Product Specific Protocols
WB protocol for RASGRP2 antibody 30189-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,CoIP

Biochim Biophys Acta Mol Cell Res

NEDD4L-mediated RASGRP2 suppresses high-glucose and oxLDL-induced vascular endothelial cell dysfunctions by activating Rap1 and R-Ras

Authors - Guozhu Chen
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