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验证数据展示

  • WB analysis using 28162-1-AP

    WB analysis using 28162-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28162-1-AP (RASEF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 28162-1-AP

    IHC staining of human stomach using 28162-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 28162-1-AP (RASEF antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RASEF antibody included
  • IHC staining of human stomach using 28162-1-AP

    IHC staining of human stomach using 28162-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 28162-1-AP (RASEF antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RASEF antibody included

RASEF Polyclonal antibody

RASEF Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28162-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, ELISA

别名

Ras-related protein Rab-45, Ras related protein Rab 45, Ras and EF-hand domain-containing protein, RAB45

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inLNCaP cells, PC-12 cells
Positive IHC detected inhuman stomach tissue, human intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy RASEF Ready-To-Use IHC Kit. RASEF primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28162-1-AP targets RASEF in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag28049

Product name: Recombinant human RASEF protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-245 aa of BC023566

Sequence: MEADGDGEELARLRSVFAACDANRSGRLEREEFRALCTELRVRPADAEAVFQRLDADRDGAITFQEFARGFLGSLRGGRRRDWGPLDPAPAVSEAGPETHDSEEDEGDEDAAAALATSCGPASPGRAWQDFQARLGDEAKFIPREEQVSTLYQNINLVEPRLIQPYEHVIKNFIREIRLQSTEMENLAIAVKRAQDKAAMQLSELEEEMDQRIQAAEHKTRKDEKRKAEEALSDLRRQYETEVGD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAS and EF-hand domain containing
别名 Ras-related protein Rab-45, Ras related protein Rab 45, Ras and EF-hand domain-containing protein, RAB45
计算分子量 740 aa, 83 kDa
观测分子量 90-100 kDa
GenBank蛋白编号BC023566
基因名称 RASEF
Gene ID (NCBI) 158158
RRIDAB_2881079
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IZ41
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RASEF, or RAS and EF-hand domain-containing protein, is a member of the Rab GTPase protein family. RASEF is a protein with significant implications in lung cancer, potentially serving as both a prognostic biomarker and a therapeutic target. Its role in cancer cell growth and its interaction with ERK1/2 signaling make it a promising candidate for further research and development of new molecular therapies in lung cancer treatment.

实验方案

Product Specific Protocols
IHC protocol for RASEF antibody 28162-1-APDownload protocol
WB protocol for RASEF antibody 28162-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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