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验证数据展示

  • WB analysis of HeLa using 22140-1-AP (same clone as 22140-1-PBS)

    WB analysis of HeLa using 22140-1-AP (same clone as 22140-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 22140-1-AP (RASAL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22140-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 22140-1-AP (same clone as 22140-1-PBS)

    IF Staining of HeLa using 22140-1-AP (same clone as 22140-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 22140-1-AP (RASAL2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22140-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 22140-1-AP (same clone as 22140-1-PBS)

    IHC staining of mouse testis using 22140-1-AP (same clone as 22140-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22140-1-AP (RASAL2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22140-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RASAL2 antibody included
  • Featured Product
  • KD/KO Validated

RASAL2 Polyclonal antibody, PBS Only

RASAL2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 22140-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Ras GTPase activating protein nGAP, Ras GTPase-activating protein nGAP, RAS protein activator like 2, RAS protein activator-like 2, RASAL 2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® RASAL2 Ready-To-Use IHC Kit. RASAL2 primary antibody included.

产品信息

22140-1-PBS targets RASAL2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag17555

Product name: Recombinant human RASAL2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 609-958 aa of BC110611

Sequence: LRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDSYFRGKTLLLVQQASSQSMTYSEKDERESSLPNGRSVSLMDLQDTHAAQVEHASVMLDVPIRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGGLQPLSFQNPVYHLNNPIPAMPKASIDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RAS protein activator like 2
别名 Ras GTPase activating protein nGAP, Ras GTPase-activating protein nGAP, RAS protein activator like 2, RAS protein activator-like 2, RASAL 2
计算分子量 1262 aa, 142 kDa
观测分子量 140-150 kDa
GenBank蛋白编号BC110611
基因名称 RASAL2
Gene ID (NCBI) 9462
RRIDAB_2879005
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UJF2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RASAL2 (RAS protein activator like 2) is a protein-coding gene that encodes a member of the RAS GTPase-activating protein (RAS-GAP) family. The encoded protein contains a characteristic GAP-related domain (GRD) that enables it to catalyze the hydrolysis of RAS-GTP to RAS-GDP, thereby functioning as a negative regulator of RAS signaling. Structurally, RASAL2 also possesses additional domains including a Pleckstrin homology (PH) domain and a C2 domain, which are involved in membrane localization and calcium/lipid binding. RASAL2 has been extensively studied for its context-dependent roles in cancer, predominantly functioning as a tumor suppressor in malignancies such as breast, bladder, renal cell, ovarian, and lung cancers, where its downregulation or loss promotes tumor growth, stemness, epithelial-mesenchymal transition (EMT), and metastasis.

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