验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22140-1-AP targets RASAL2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag17555 Product name: Recombinant human RASAL2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 609-958 aa of BC110611 Sequence: LRFLCPAIMSPSLFNLMQEYPDDRTSRTLTLIAKVIQNLANFAKFGNKEEYMAFMNDFLEHEWGGMKRFLLEISNPDTISNTPGFDGYIDLGRELSVLHSLLWEVVSQLDKATVAKLGPLPRVLADITKSLTNPTPIQQQLRRFTEHNSSPNVSGSLSSGLQKIFEDPTDSDLHKLKSPSQDNTDSYFRGKTLLLVQQASSQSMTYSEKDERESSLPNGRSVSLMDLQDTHAAQVEHASVMLDVPIRLTGSQLSITQVASIKQLRETQSTPQSAPQVRRPLHPALNQPGGLQPLSFQNPVYHLNNPIPAMPKASIDSSLENLSTASSRSQSNSEDFKLSGPSNSSMEDFT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RAS protein activator like 2 |
| 别名 | nGAP, RAS protein activator like 2, RASAL2 |
| 计算分子量 | 1262 aa, 142 kDa |
| 观测分子量 | 140-150 kDa |
| GenBank蛋白编号 | BC110611 |
| 基因名称 | RASAL2 |
| Gene ID (NCBI) | 9462 |
| RRID | AB_2879005 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UJF2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RASAL2 (Ras protein activator like 2), also known as nGAP, is a Ras GTPase-activating protein, containing a GRD domain and a coiled-coil structure at the C terminus. It is able to complement the defective RasGAP function in a yeast system. RASAL2 is highly homologous to Ras-specific GTPase activating proteins (RasGAPs). Two transcript variants exist for RASAL2 due to alternative splicing events.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RASAL2 antibody 22140-1-AP | Download protocol |
| IF protocol for RASAL2 antibody 22140-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Metab Rasal2 deficiency reduces adipogenesis and occurrence of obesity-related disorders.
| ||
J Clin Transl Hepatol RASAL2 Deficiency Attenuates Hepatic Steatosis by Promoting Hepatic VLDL Secretion via the AKT/TET1/MTTP Axis
| ||
Int J Biol Sci RASAL2 mediated the enhancement of YAP1/TIAM1 signaling promotes malignant phenotypes of pancreatic ductal adenocarcinoma
| ||
J Transl Med Rasal2 inhibits autophagic-exosomes secretion via regulating Rab27a in triple-negative breast cancer progression | ||
Transl Res RAS protein activator-like 2 (RASAL2) initiates peritubular capillary rarefaction in hypoxic renal interstitial fibrosis |