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  • Featured Product
  • KD/KO Validated

RASAL2 Polyclonal antibody

RASAL2 Polyclonal Antibody for IF/ICC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IF/ICC, WB,ELISA

Conjugate

Unconjugated

Cat No : 22140-1-AP

Print datasheet

Synonyms

nGAP, RAS protein activator like 2, RASAL2

验证数据展示

  • WB analysis of HeLa using 22140-1-AP

    WB analysis of HeLa using 22140-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 22140-1-AP (RASAL2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 22140-1-AP

    IF Staining of HeLa using 22140-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 22140-1-AP (RASAL2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 22140-1-AP is RASAL2 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivitymouse
Immunogen RASAL2 fusion protein Ag17555 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAS protein activator like 2
Synonyms nGAP, RAS protein activator like 2, RASAL2
Calculated Molecular Weight 1262 aa, 142 kDa
Observed Molecular Weight 140-150 kDa
GenBank Accession NumberBC110611
Gene Symbol RASAL2
Gene ID (NCBI) 9462
RRIDAB_2879005
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UJF2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RASAL2 (Ras protein activator like 2), also known as nGAP, is a Ras GTPase-activating protein, containing a GRD domain and a coiled-coil structure at the C terminus. It is able to complement the defective RasGAP function in a yeast system. RASAL2 is highly homologous to Ras-specific GTPase activating proteins (RasGAPs). Two transcript variants exist for RASAL2 due to alternative splicing events.

实验方案

Product Specific Protocols
WB protocol for RASAL2 antibody 22140-1-APDownload protocol
IF protocol for RASAL2 antibody 22140-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Metab

Rasal2 deficiency reduces adipogenesis and occurrence of obesity-related disorders.

Authors - Xiaoqiang Zhu
  • KD Validated
View Article