• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

RAPH1 Polyclonal antibody

RAPH1 Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 25750-1-AP

Print datasheet

Synonyms

ALS2CR18, ALS2CR9, KIAA1681, Lamellipodin, LPD, PREL 2, PREL2, Proline rich EVH1 ligand 2, Protein RMO1, RalGDS/AF 6, RAPH1, RMO1

验证数据展示

  • WB analysis of SKOV-3 using 25750-1-AP

    WB analysis of SKOV-3 using 25750-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 25750-1-AP (RAPH1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 25750-1-AP

    IF Staining of HepG2 using 25750-1-AP

    Immunofluorescent analysis of HepG2 cells using 25750-1-AP (RAPH1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inSKOV-3 cells
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25750-1-AP targets RAPH1 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity human
Immunogen RAPH1 fusion protein Ag22705 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Ras association (RalGDS/AF-6) and pleckstrin homology domains 1
Synonyms ALS2CR18, ALS2CR9, KIAA1681, Lamellipodin, LPD, PREL 2, PREL2, Proline rich EVH1 ligand 2, Protein RMO1, RalGDS/AF 6, RAPH1, RMO1
Calculated Molecular Weight 1250 aa, 135 kDa
Observed Molecular Weight 67 kDa
GenBank Accession NumberBC156922
Gene Symbol RAPH1
Gene ID (NCBI) 65059
RRIDAB_2880223
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ70E73
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAPH1 encodes the lamellipodin protein (Lpd), which binds to Ena and VASPs to assist in actin cytoskeleton assembly; in this way, the gene plays an important role in cellular motility and lamelipodial protrusion. This protein has 9 isoforms producing different molecular weights of 135 kDa, 67 kDa, 70 kDa, 73 kDa.

实验方案

Product Specific Protocols
WB protocol for RAPH1 antibody 25750-1-APDownload protocol
IF protocol for RAPH1 antibody 25750-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols