验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells, Jurkat cells, K-562 cells, MOLT-4 cells, U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60940-1-Ig targets RAP1GDS1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag32728 Product name: Recombinant human RAP1GDS1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 176-406 aa of BC001851 Sequence: QLINMGVIPTLVKLLGIHCQNAALTEMCLVAFGNLAELESSKEQFASTNIAEELVKLFKKQIEHDKREMIFEVLAPLAENDAIKLQLVEAGLVECLLEIVQQKVDSDKEDDITELKTGSDLMVLLLLGDESMQKLFEGGKGSVFQRVLSWIPSNNHQLQLAGALAIANFARNDANCIHMVDNGIVEKLMDLLDRHVEDGNVTVQHAALSALRNLAIPVINKAKMLSAGVTE 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | RAP1, GTP-GDP dissociation stimulator 1 |
| 别名 | Exchange factor smgGDS, Rap1 GTPase-GDP dissociation stimulator 1, SMG GDS protein, SmgGDS |
| 计算分子量 | 66 kDa |
| 观测分子量 | 55-66 kDa |
| GenBank蛋白编号 | BC001851 |
| 基因名称 | RAP1GDS1 |
| Gene ID (NCBI) | 5910 |
| RRID | AB_3743250 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P52306 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GDS stimulates the GDP/GTP exchange reaction of a group of small GTP-binding proteins (G proteins), including at least c-Ki-ras p21, smg p21A, smg p21B, rhoA p21 and rhoB p21, by stimulating the dissociation of GDP from and the subsequent binding of GTP to each small G protein. The small GTPase Rap1, which is activated by a large variety of stimuli, functions in the control of integrin-mediated cell adhesion. RAP1GDS1 (synonym: GDS1)is one of RAP1 GTP-GDP dissociation stimulators.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RAP1GDS1 antibody 60940-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
