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验证数据展示

  • WB analysis using 83742-5-RR

    WB analysis using 83742-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83742-5-RR (RAGE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse lung using 83742-5-RR

    IHC staining of mouse lung using 83742-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 83742-5-RR (Ager/Rage antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse lung using 83742-5-RR

    IF Staining of mouse lung using 83742-5-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using Ager/Rage antibody (83742-5-RR, Clone: 240774F11 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83742-5-RR

    Affinity and Kinetic Characterization of 83742-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83608-4-RR against Human ATM were performed. The affinity constant is 2.58 nM. Biolayer interferometry (BLl) kinetic assays of 83608-4-RR against Mouse RAGE were performed. The affinity constant is 6.41 nM.

RAGE Recombinant antibody

RAGE Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 83742-5-RR

产品说明书

CloneNo. 240774F11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

Ager, RAGE1, RAGE 1, MOK, 240774F11

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inrat lung tissue, mouse lung tissue
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse lung tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83742-5-RR targets RAGE in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 advanced glycosylation end product-specific receptor
别名 Ager, RAGE1, RAGE 1, MOK, 240774F11
计算分子量43 kDa
观测分子量42-55 kDa
GenBank蛋白编号NM_007425.3
基因名称 Ager
Gene ID (NCBI) 11596
RRIDAB_3671340
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ62151-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The receptor for advanced glycation end products (RAGE) is a cell surface transmembrane multiligand receptor, also known as Ager, is a multiligand member of the immunoglobulin superfamily. It has many isoforms and is predominantly expressed in the lung. AGER expression is upregulated in many diseases such as Alzheimer's disease, diabetes mellitus, atherosclerosis, and rheumatoid arthritis (PMID: 35099614).

实验方案

Product Specific Protocols
WB protocol for RAGE antibody 83742-5-RRDownload protocol
IHC protocol for RAGE antibody 83742-5-RRDownload protocol
IF protocol for RAGE antibody 83742-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols