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Host
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Species Reactivity
0
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0
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0
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 83742-5-RR (same clone as 83742-5-PBS)

    WB analysis using 83742-5-RR (same clone as 83742-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83742-5-RR (RAGE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83742-5-PBS in a different storage buffer formulation.

  • IHC staining of mouse lung using 83742-5-RR (same clone as 83742-5-PBS)

    IHC staining of mouse lung using 83742-5-RR (same clone as 83742-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 83742-5-RR (Ager/Rage antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83742-5-PBS in a different storage buffer formulation.

  • IF Staining of mouse lung using 83742-5-RR (same clone as 83742-5-PBS)

    IF Staining of mouse lung using 83742-5-RR (same clone as 83742-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using Ager/Rage antibody (83742-5-RR, Clone: 240774F11 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83742-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83742-5-RR

    Affinity and Kinetic Characterization of 83742-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83608-4-RR against Human ATM were performed. The affinity constant is 2.58 nM. Biolayer interferometry (BLl) kinetic assays of 83608-4-RR against Mouse RAGE were performed. The affinity constant is 6.41 nM.

Mouse RAGE Recombinant antibody, PBS Only

RAGE Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 83742-5-PBS

产品说明书

CloneNo. 240774F11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

Ager, RAGE1, RAGE 1, MOK, 240774F11

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83742-5-PBS targets RAGE in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 advanced glycosylation end product-specific receptor
别名 Ager, RAGE1, RAGE 1, MOK, 240774F11
计算分子量43 kDa
观测分子量42-55 kDa
GenBank蛋白编号NM_007425.3
基因名称 Ager
Gene ID (NCBI) 11596
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ62151-1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The receptor for advanced glycation end products (RAGE) is a cell surface transmembrane multiligand receptor, also known as Ager, is a multiligand member of the immunoglobulin superfamily. It has many isoforms and is predominantly expressed in the lung. AGER expression is upregulated in many diseases such as Alzheimer's disease, diabetes mellitus, atherosclerosis, and rheumatoid arthritis (PMID: 35099614).